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Purified anti-human CD174 Antibody

Pricing & Availability
Clone
NCRC37 (See other available formats)
Regulatory Status
RUO
Other Names
SC104
Isotype
Mouse IgG1, κ
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Product Citations
publications
NCRC37_Pure_CD174_Antibody_1_020718
Human colorectal tumor cell line COLO205 was stained with purified CD174 (clone NCRC37, filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by Goat anti-mouse IgG FITC (clone Poly4053).
  • NCRC37_Pure_CD174_Antibody_1_020718
    Human colorectal tumor cell line COLO205 was stained with purified CD174 (clone NCRC37, filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by Goat anti-mouse IgG FITC (clone Poly4053).
  • NCRC37_Pure_CD174_Antibody_2_020718
    Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD174 (clone NCRC37) overnight at 4°C. On the next day, tissue was incubated with Alexa Fluro® 594 goat anti-mouse IgG (Clone Poly4054) antibody (red) for an hout at room temperature. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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Description

CD174 is also known as Lewis Y, Fucosyltransferase 3, Galactoside 3(4)-L-fucosyltransferase which is encoded by FUT3 gene. FUT3 protein has 361a.a., around 39.7 kD and is highly expressed on duodenum and small intestine. The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Tissue cultured colorectal tumor cell line (C170, C146, COLO205, and JW)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Durrant, LG. et al. 2006. Cancer Res. 66:5901-9
RRID
AB_2728417 (BioLegend Cat. No. 392602)

Antigen Details

Distribution

Highly expressed on glandular cells of duodenum and small intestine. 

Function
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme.
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Durrant, LG. et al. 2006. Cancer Res. 66:5901-9
Gene ID
2525 View all products for this Gene ID
UniProt
View information about CD174 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02/09/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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