- Clone
- SJ25C1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD19 Molecule, CD19 Antigen, Differentiation Antigen CD19, B-Lymphocytes Surface Antigen B4, T-cell Surface Antigen Leu-12, CVID3, B4, B-lymphocyte Antigen CD19
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_SJ25C1_PURE_CD19_FC_081214.jpg A_SJ25C1_PURE_CD19_FC_081214.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SJ25C1_Purified_CD19_Antibody_FC_081214.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes were stained with purified CD19 (clone SJ25C1, closed histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE. -
Frozen human tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-human CD19 (clone SJ25C1) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 594 anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
363001 | 25 µg | $54 | ||||
363002 | 100 µg | $112 |
CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NALM1 + NALM16 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone SJ25C1 partially blocks anti-human CD19 clones HIB19 and 4G7 staining based on in-house testing
- Product Citations
-
- RRID
-
AB_2563988 (BioLegend Cat. No. 363001)
AB_2563989 (BioLegend Cat. No. 363002)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 95 kD.
- Distribution
-
B lineage (except plasma cells), follicular dendritic cells.
- Function
- B cell activation and differentiation.
- Interaction
- Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells and interacts with VAV and LYN. It also interacts with GRAB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378 and with PLCG2 when phosphorylated on Tyr-409.
- Ligand/Receptor
- Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR co-receptor.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915. - Gene ID
- 930 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
Other Formats
View All CD19 Reagents Request Custom ConjugationCustomers Also Purchased
![](/Files//Images/media_assets/products/product_images/HIB19_Purified_061807.jpg)
![](/Files//Images/media_assets/products/product_images/2H7_Purified_021805.jpg)
![](/Files//Images/media_assets/products/product_images/SK3_PE_102809.jpg)
![](/Files//Images/media_assets/products/product_images/HI30_Purified_071307.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Brilliant Violet 421™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... Frozen human tonsil section was fixed with 4% paraformaldehy... -
Purified anti-human CD19
Human peripheral blood lymphocytes were stained with purifie... Frozen human tonsil section was fixed with 4% paraformaldehy... -
PE anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
APC anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
FITC anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
APC/Cyanine7 anti-human CD19
Human peripheral blood lymphocytes were stained with CD20 FI... -
PE/Cyanine7 anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
PerCP anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
PerCP/Cyanine5.5 anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Brilliant Violet 510™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Brilliant Violet 711™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Brilliant Violet 605™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Brilliant Violet 650™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Brilliant Violet 785™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
APC/Fire™ 750 anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 PE ... -
PE/Dazzle™ 594 anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Alexa Fluor® 700 anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Pacific Blue™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Alexa Fluor® 488 anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Alexa Fluor® 647 anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 PE ... Frozen human tonsil section was fixed with 4% paraformaldehy... -
PE/Cyanine5 anti-human CD19
Human peripheral blood lymphocytes were stained with CD20 (c... -
Spark YG™ 593 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark YG™ 581 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 574 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 550 anti-human CD19 (Flexi-Fluor™)
-
PE/Fire™ 640 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us