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Purified anti-human CD1d Antibody

Pricing & Availability
Clone
51.1 (See other available formats)
Regulatory Status
RUO
Other Names
R3G1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
51.1_PE__1_090310
Human peripheral blood lymphocytes stained with CD19 FITC and purified CD1d (51.1) conjugated with PE (top) or mouse IgG2b PE isotype control (bottom)
  • 51.1_PE__1_090310
    Human peripheral blood lymphocytes stained with CD19 FITC and purified CD1d (51.1) conjugated with PE (top) or mouse IgG2b PE isotype control (bottom)
  • 51.1_PE__2_090310
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350302 100 µg $106
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Description

CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to iNKT cells analogous to MHC molecule presentation of peptides to T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD1d-Fc fusion
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP, Stim, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.

Application References

(PubMed link indicates BioLegend citation)
  1. Exley M, et al. 2000. Immunology 100:37. (IHC, WB)
  2. Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB)
  3. Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
Product Citations
  1. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  2. Frasca D, et al. 2020. Front Immunol. 11:392. PubMed
  3. Guo T, et al. 2018. J Immunol. 200:500. PubMed
RRID
AB_10641848 (BioLegend Cat. No. 350302)

Antigen Details

Structure
MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution

Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells

Function
Present lipid antigens to iNKT cells
Ligand/Receptor
When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens, TCRs
Antigen References

1. Koch M, et al. 2005. Nat. Immunol. 6:819.
2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010.
3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889.
4. Teige A, et al. 2010. J. Immunol. 185:345.

Gene ID
912 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org
Go To Top Version: 3    Revision Date: 08/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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