- Clone
- B1/35 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD248 molecule, Tumor endothelial marker 1(TEM1), CD164 sialomucin-like 1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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379102 | 100 µg | $335 |
Endosialin, also known as Tumor Endothelial Marker 1 (TEM1), CD248 and CD164 sialomucin-like 1 (CD164L1), is classified as a C-type lectin-like protein and shares both sequence and structural 39% homology with thrombomodulin (CD141) and 33% homology to complement receptor C1qRp (CD93). CD248 is expressed on pericytes and fibroblasts during tissue development, tumor neovascularization and inflammation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Diploid human fibroblasts
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
1. Teicher BA. 2019. Oncotarget. 10:993-1009. (Flow, ICC)
2. Hardie DL, et al. 2011. Immunology. 133:288-95. (IHC-P, Flow) - RRID
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AB_2927975 (BioLegend Cat. No. 379102)
Antigen Details
- Structure
- Heavily glycosylated, single-pass transmembrane protein
- Distribution
-
Endosialin is expressed on pericytes and fibroblasts during tissue development, tumor neovascularization and inflammation.
- Cell Type
- B cells, Dendritic cells, Fibroblasts, Mesenchymal Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Bagley RG, et al. 2009. Int J Oncol. 34:619-27.
- Teicher BA. 2019. Oncotarget. 10:993-1009.
- Teicher BA. 2007. Int J Oncol. 2007. 30:305-12.
- Rouleau C, et al. 2008. Clin Cancer Res. 14:7223-36.
- Gene ID
- 57124 View all products for this Gene ID
- UniProt
- View information about CD248 on UniProt.org
Other Formats
View All CD248 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD248 (Endosialin) | B1/35 | FC,IHC-P,ICC |
TotalSeq™-C1458 anti-human CD248 (Endosialin) | B1/35 | PG |
TotalSeq™-A1458 anti-human CD248 (Endosialin) | B1/35 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD248 (Endosialin)
Human LWB were stained with anti-human CD8 APC and purified ... Human bone marrow-derived mesenchymal stem cells were staine... Human paraffin-embedded breast tissue slices were prepared w... Human bone marrow-derived mesenchymal stem cells were fixed ... -
TotalSeq™-C1458 anti-human CD248 (Endosialin)
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TotalSeq™-A1458 anti-human CD248 (Endosialin)
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