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Purified anti-human CD88 (C5aR) Antibody

Pricing & Availability
Clone
S5/1 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1. S5slash1_PE_092809
Human peripheral blood granulocytes stained with S5/1 PE
  • 1. S5slash1_PE_092809
    Human peripheral blood granulocytes stained with S5/1 PE
  • 2. S5-1_PURE_CD88_Antibody_072722
    IHC staining of purified anti-CD88 (C5aR) (clone S5/1) on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using 1X Tris-EDTA pH9.0, the tissue was incubated with (B) or without (A) 1.0 μg/mL of the primary antibody overnight at 4°C. This was followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801) and the slide was mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
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344302 100 µg $129
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Description

CD88 is a single chain protein with seven membrane-spanning regions and has a MW of 43 kD. CD88 is expressed by monocytes, neutrophils and eosinophils. Also, there have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release and superoxide anion production.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant peptide huC5aR N-terminal -NT (Asp15-Asp27)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S5/1 blocks the binding of C5a to CD88.

Application References

(PubMed link indicates BioLegend citation)
  1. Kiener HP, et al. 1998. Arthritis Rheum. 41:233.
  2. Elsner J, et al. 1994. Blood 83:3324.
  3. Oppermann M, et al. 1993. J. Immunol. 151:3785.
Product Citations
  1. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  2. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  3. Brandolini L, et al. 2022. Cell Death Dis. 13:500. PubMed
  4. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  5. Colley CS, et al. 2018. MAbs. 10:104. PubMed
  6. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  7. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  8. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_2259318 (BioLegend Cat. No. 344302)

Antigen Details

Structure
CD88 is a single chain protein with seven membrane-spanning regions and a MW of 43 kD.
Distribution

CD88 is expressed by monocytes, neutrophils and eosinophils. There has been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells.

Function
After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction
CD88 is coupled to heterotrimeric G proteins such as Gi. The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Cell Type
Eosinophils, Monocytes, Neutrophils
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol Chem. 284:12363.
3. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
4. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
5. Buck E and Wells JA. et al. 2005. P. Natl. Acad. Sci. USA 102:2719.

Gene ID
728 View all products for this Gene ID
UniProt
View information about CD88 on UniProt.org
Go To Top Version: 3    Revision Date: 08/31/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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