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Purified anti-human CXCL10 (IP-10) Antibody

Pricing & Availability
Clone
J034D6 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon gamma induced protein-10 (IP-10)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
J034D6_PURE_CXCL10_Antibody_1_ICFC_030714
IFN-γ (1500U/ml)-stimulated (24 hours) human peripheral blood monocytes (in the presence of monensin) were fixed, permeabilized, and then stained with CD14 (clone HCD14) APC and purified CXCL10 (clone J034D6, top) or mouse IgG2a, κ isotype control, followed by anti-mouse IgG2a PE.
  • J034D6_PURE_CXCL10_Antibody_1_ICFC_030714
    IFN-γ (1500U/ml)-stimulated (24 hours) human peripheral blood monocytes (in the presence of monensin) were fixed, permeabilized, and then stained with CD14 (clone HCD14) APC and purified CXCL10 (clone J034D6, top) or mouse IgG2a, κ isotype control, followed by anti-mouse IgG2a PE.
  • J034D6_PURE_CXCL10_Antibody_2_ICFC_030714
Compare all formats See supplemental WB data...
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519501 50 µg $101
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519502 500 µg $347
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Description

CXCL10, also known as IP-10, is a 8.7 kD chemokine. It belongs to the CXC chemokine family. It is expressed by monocytes, fibroblasts, and endothelial cells in response to IFNγ. It can interact with CXCR3 and plays an important role in immune cell trafficking, particularly of T cells, dendritic cells, and NK cells. In addition, it alters expression of adhesion molecules.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  2. Kang SH, et al. 2018. Oncoimmunology. 8:e1515057. PubMed
RRID
AB_2563205 (BioLegend Cat. No. 519501)
AB_2563205 (BioLegend Cat. No. 519502)

Antigen Details

Structure
CXC chemokine family, 8.7 kD
Distribution

Monocytes, endothelial cells, and fibroblasts

Function
Interacts with CXCR3 and serves as a chemoattractant for T cells, dendritic cells, monocytes/macrophages, and NK cells
Ligand/Receptor
CXCR3
Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Luster AD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:2868. 
2. Kaplan G, et al. 1987. J. Exp. Med. 166:1098. 
3. Sarris AH, et al. 1993. J. Exp. Med. 178:1127.

Gene ID
3627 View all products for this Gene ID
UniProt
View information about CXCL10 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/07/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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