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Purified anti-human FLT3L Antibody

Pricing & Availability
Clone
W19353A (See other available formats)
Regulatory Status
RUO
Other Names
FLT3LG, Fms related receptor tyrosine kinase 3 ligand, Fms-like tyrosine kinase 3 ligand, Flt3 Ligand, SL cytokine
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19353A_Purified_FLT3L_FC_081924
Recombinant human IL-7 (Cat. No. 581902) stimulated (15 ng/mL for 3 days) human peripheral blood mononuclear cells were stained with anti-human CD3 (clone UCHT1) APC and purified anti-human FLT3L (clone W19353A) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
  • W19353A_Purified_FLT3L_FC_081924
    Recombinant human IL-7 (Cat. No. 581902) stimulated (15 ng/mL for 3 days) human peripheral blood mononuclear cells were stained with anti-human CD3 (clone UCHT1) APC and purified anti-human FLT3L (clone W19353A) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
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345602 100 µg $422
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Description

Human FLT3L was initially cloned from a T cell cDNA library using a mouse probe; the human and mouse FLT3L proteins share 72% amino acid identity. FLT3L is synthesized as a type I membrane-bound protein, which is cleaved to become a soluble growth factor. An additional soluble form of FLT3L has been reported as a result of alternative splicing. TACE (ADAM17) plays a key role in the ectodomain shedding of FLT3L. In fact, serum FLT3L levels are decreased in TACE deficient mice.

FLT3L is crucial for the development of the two main subsets of dendritic cells (DCs): conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Changes in the development or number of DCs can alter T cell immunity and tolerance. A feedback loop between DCs and Tregs is regulated via FLT3L, as it has been shown that the increase in Tregs induced by DC expansion delays the onset of type 1 autoimmune diabetes and IBD in mice. FLT3L also facilitates formation of Tregs and thus, reduces severity of antigen-induced arthritis in mice. FLT3L deficiency in humans has been shown to result in bone marrow hypoplasia, with low levels of hematopoietic progenitors (particularly myeloid and B cell progenitors). FLT3L is elevated in the synovial fluid of rheumatoid arthritis (RA) patients and FLT3L has been included in panels of preclinical markers for predicting the possible development of RA. The innate sensing pathway triggered by Plasmodium infection regulates DC homeostasis and adaptive immunity via FLT3L release. High levels of FLT3L and increased DCs have been detected in humans and mice during Plasmodium infection.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human FLT3L transfected cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Surface staining in fixed cells may result in lower signal compared to unfixed cells.

Antigen Details

Structure
P49771
Distribution

Hematopoietic precursors, stromal bone marrow cells, T Lymphocytes

Function
FLT3L stimulates the proliferation of hematopoietic precursors and development of B cells and dendritic cells. Additionally, FLT3L is also required for the development of monocytes in humans and NK cells in mice. FLT3L regulates the homeostatic feedback loop between DCs and Tregs.
Interaction
Hematopoietic stem progenitor cells, progenitor cells, DCs, monocytes, B cells, and NK cells
Ligand/Receptor
Fms related receptor tyrosine kinase 3, FLT3, CD135
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Hannum C, et al. 1994. Nature. 368:643-8.
  2. Waskow C. 2008. Nat Immunol. 9:676-83.
  3. Darrasse-Jèse G, et al. 2009. J Exp Med. 206:1853-62.
  4. Horiuchi K, et al. 2009. J Immunol. 182:7408-14.
  5. Svensson MN, et al. 2013. PLoS One. 8:e54884.
  6. Guermonprez P, et al. 2013. Nat Med. 19:730-8.
  7. Chklovskaia E, et al. 2001. Blood. 97:1027-34.
  8. Momenilandi M, et al. 2024. Cell. 187:2817.
Gene ID
2323 View all products for this Gene ID
UniProt
View information about FLT3L on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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