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Purified anti-human IFN-β Antibody

Pricing & Availability
Clone
IFNb/A1 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor, IFN-b, IFN-beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
IFNbA1_PURE_IFN-Beta_Antibody_1_WB_101716
Recombinant human IFN-β (50 ng, lane 1) and recombinant mouse IFN- β (50 ng, lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-human IFN-β (clone IFNb/A1) antibody. Proteins were visualized using a Goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • IFNbA1_PURE_IFN-Beta_Antibody_1_WB_101716
    Recombinant human IFN-β (50 ng, lane 1) and recombinant mouse IFN- β (50 ng, lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-human IFN-β (clone IFNb/A1) antibody. Proteins were visualized using a Goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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514002 200 µg $293
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Description

The IFNb/A1 clone recognizes natural human IFN-β and recombinants β1a and β1b.It is capable of neutralizing IFN-β.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant HuIFN-β1b
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ELISA, Neut - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-ß bioactivity (Cat. No. 514003).

Additional reported applications (for the relevant formats) include: ELISA1.

Epitope: Human IFN-β site I, core peptide amino acid residues 32-56, AB loop (AB2, AB3)

Application References

(PubMed link indicates BioLegend citation)
  1. Redlich PN and Grossberg SE, et al. 1989. J. Immunol. 143:1887. (ELISA, Neut)
  2. Redlich PN, et al. 1991. P. Natl. Acad. Sci. USA 88:4040.
  3. Runkel L, et al. 2001. IFN & Cytokine Res. 21:931.
Product Citations
  1. Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed
  2. Shemesh M, et al. 2021. PLoS Pathog. 17:e1009800. PubMed
  3. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
RRID
AB_2122765 (BioLegend Cat. No. 514002)

Antigen Details

Structure
Interferon; contains 187 amino acids with predicted molecular weight 22 kD.
Bioactivity
Non-specific humoral immune responses and immune responses against viral infections, increases expression of MHC class I antigens, blocks expression of MHC class II antigens stimulated by IFN-γ
Cell Sources
Fibroblasts, epithelial cells
Cell Targets
Broad, includes NK cells, T suppressor cells, activated monocytes
Receptors
IFNα/βR (IFNAR-1, IFNAR-2)
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagarajan U, 2011. Crit. Rev. Immunol. 31:459.
2. Honda K, et al. 2005. Int. Immunol. 17:1367.

Regulation
Upregulated by viruses, double-stranded RNA, micro-organisms, TNF, IL-1
Gene ID
3456 View all products for this Gene ID
UniProt
View information about IFN-beta on UniProt.org
Go To Top Version: 6    Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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