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Purified anti-human IL-7 Antibody

Pricing & Availability
Clone
BVD10-40F6 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-7, Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growth factor
Isotype
Rat IgG1, κ
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Product Citations
publications
Human_IL-7_TMB_020405
  • Human_IL-7_TMB_020405
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501302 500 µg $297
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Description

IL-7 is a potent lymphoid cell growth factor which promotes the proliferation of pre-B cells, thymocytes, T cell progenitors, and mature T cells. IL-7 induces formation of LAK cells and CTLs, and can stimulate tumoricidal activity of monocytes and macrophages. IL-7 can induce upregulation of proinflammatory cytokines in myeloid cells. IL-7 is expressed by stromal cells. The BVD10-40F6 antibody can neutralize the bioactivity of natural or recombinant IL-7.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed, recombinant human IL-7
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-7 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1,2: The purified BVD10-40F6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-7 antibody (Cat. No. 506602) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1: The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-7 bioactivity (Cat. No. 501305 & 501306).
Additional reported applications (for the relevant formats) include: Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20.
Product Citations
  1. Eberhardt F, et al. 2023. Int J Mol Med. 52:. PubMed
  2. Wallington JC, et al. 2018. J Allergy Clin Immunol. 2182:141. PubMed
  3. Haug V, et al. 2020. Transplantation. e260:104. PubMed
  4. Gilboa T, et al. 2020. Adv Healthc Mater. e2001111:. PubMed
RRID
AB_315164 (BioLegend Cat. No. 501302)

Antigen Details

Structure
Cytokine; 20-28 kD (Mammalian)
Bioactivity
Proliferation of pre-/pro-B cells, thymocytes, T cells; formation of LAK cells, CTLs, stimulation of tumoricidal activity monocytes/macrophages; upregulate proinflammatory cytokines in myeloid cells
Cell Sources
Adherent bone marrow stromal cells, thymic stromal cells, spleen, keratinocytes
Cell Targets
Progenitor B and T cells; CD4-CD8- thymocytes; megakaryocytes
Receptors
Heterodimer IL-7Rα (CD127); γ-subunit (CD132) in common with IL-2R, IL-4R, IL-13R, IL-15R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Park L, et al. 1992. Adv. Exp. Med. Biol. 323 125.
3. Melchers F, et al. 1992. Adv. Exp. Med. Biol. 323 111.

Regulation
Downregulated by TGF-β
Gene ID
3574 View all products for this Gene ID
UniProt
View information about IL-7 on UniProt.org

Other Formats

View All IL-7 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IL-7 BVD10-40F6 ELISA Capture
Ultra-LEAF™ Purified anti-human IL-7 BVD10-40F6 ELISA Capture,ELISPOT Capture,Neut,WB
Go To Top Version: 2    Revision Date: 06/28/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human IL-7

    Human_IL-7_TMB_020405

  • Ultra-LEAF™ Purified anti-human IL-7

    HumanIL-7
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