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Purified anti-human Mesothelin Antibody

Pricing & Availability
Clone
MN (See other available formats)
Regulatory Status
RUO
Other Names
MSLN, CAK1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
Human_Mesothelin_Antibody_ELISA_022516
  • Human_Mesothelin_Antibody_ELISA_022516
  • Human_Mesothelin_Antibody_ELISA_2_032322
    HeLa cells (positive target) were stained with 0.25 µg/test of purified anti-mesothelin antibody (clone MN) (filled histogram) or 0.25 µg/test of purified mouse IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400202) (open histogram) followed by PE goat anti-mouse IgG antibody (Cat. No. 405307).
  • Human_Mesothelin_Antibody_ELISA_3_032322
    PC-3 cells (negative target) were stained with 0.25 µg/test of purified anti-mesothelin antibody (clone MN) (filled histogram) or 0.25 µg/test of purified mouse IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400202) (open histogram) followed by PE goat anti-mouse IgG antibody (Cat. No. 405307).
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530101 50 µg $147
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Description

Human mesothelin, also known as CAK1 and MSLN, is a cell-surface protein that is expressed on normal mesothelial cells lining the pleura, pericardium, and peritoneum. It is expressed as a 70 kD pre-cursor protein with a furin cleavage site, which produces two products: 1) megakaryocyte potentiating factor (MPF) (30 kD) and 2) mesothelin (40 kD). MPF is a cytokine that is released. Mesothelin, for the most part, is membrane-bound by a glycosylphosphatidylinositol linkage and has three glycosylation sites. Soluble mesothelin is believed to result from proteolytic cleavage to release it from the membrane linkage, or from alternative splicing of the mesothelin gene.

The normal function of mesothelin is not known. Mice knockouts produced no obvious phenotypes with normal growth and reproductive parameters. However, mesothelin is overexpressed by cancer cells, particularly in mesotheliomas, ovarian adenocarcinomas, pancreatic adenocarcinomas, and squamous cell carcinomas, and lung cancers. It binds to MUC16/CA-125, which is a cell surface mucin and ovarian cancer biomarker, and has been speculated to be involved in peritoneal dissemination of ovarian cancer cells. Mesothelin has also been shown to be elevated in the serum of cancer patients compared to normal patients. As such, it has become the focus of cancer research in the areas of diagnostics, immunotherapy, and cancer vaccinology. For example, SS1P is a recombinant immunotoxin which targets mesothelin-expressing cancer cells. Its anti-mesothelin Fγ domain confers specificity, while its Pseudomonas exotoxin domain results in cell death. This potential drug has already finished Stage I clinical trials.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DNA immunization (mesothelin expression plasmid) with mesothelin-Fc fusion protein for final boost.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

FC - Verified
ELISA Detection, FC, IHC-P, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 2 µg/ml of the capture antibody was utilized to generate the example standard curve for human mesothelin. For flow cytometric staining, the suggested use of this reagent is 0.25 - 1.0 µg per million cells in 100 µL volume. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: To measure human mesothelin, this antibody can be used as a capture antibody in sandwich ELISA format, and paired with the biotinylated (clone MB) antibody (Cat. No. 520203) as the detection antibody. Recombinant Human Mesothelin (ELISA Std.) (Cat. No. 593209) can be used as the protein standard.

Note: For testing human mesothelin in serum, plasma, or supernatant, LEGEND MAX™ Human Mesothelin ELISA Kit (Cat. No. 438607 & 438608) is specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Onda M, et al. 2005. Clin. Cancer Res. 11:5840.
RRID
AB_2571908 (BioLegend Cat. No. 530101)

Antigen Details

Structure
~40 kD glycoprotein that can be membrane-bound, or relased from the membrane in a soluble form.
Distribution

Mesothelial cells lining the pleura, peritoneum, and pericardium. It is also located in various cancer cells.

Function
Not known. May promote carcinogenesis and metastasis.
Interaction
Various cancer cells such as ovarian and pancreatic cancer cells.
Ligand/Receptor
CA125 (MUC16).
Biology Area
Cancer Biomarkers, Cell Adhesion, Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pastan I, et al. 2014. Cancer Res. 74:2907.
2. Hellstrom I, et al. 2011. Expert Opin. Med. Diagn. 1:227.
3. Hassan R, et al. 2008. Eur. J. Cancer 44:46.
4. Hassan R, et al. 2006. Clin. Cancer Res. 2:447.
5. Onda M, et al. 2005. Clin. Cancer Res. 16:5840-6.
6. Hassan R, et al. 2004. Clin. Cancer Res. 10:3937.
7. Scholler N, et al. 1999. Proc. Natl. Acad. Sci. 96:11531.

Gene ID
10232 View all products for this Gene ID
UniProt
View information about Mesothelin on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Mesothelin Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human Mesothelin MN ELISA Capture,ELISA Detection,FC,IHC-P,WB
Go To Top Version: 2    Revision Date: 03/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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