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Purified anti-human/mouse ZAP-70 Antibody

Pricing & Availability
Clone
1E7.2 (See other available formats)
Regulatory Status
RUO
Other Names
Zeta chain associated protein kinase, SYK related tyrosine kinase
Isotype
Mouse IgG1, κ
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Product Citations
publications
Zap-70_080304
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • Zap-70_080304
    Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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313402 100 µg $183
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Description

ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
GST-fusion protein corresponding to residues 282-307 of human ZAP-70
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC, WB - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis or Western blotting. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, the suggested working dilution(s)is 0.5-1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2.

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC)
  2. Crespo M, et al. 2003. New Engl. J. Med. 348:1764. (WB IP)
  3. Jamroziak K, et al. 2009. Cancer Epidemiol Biomarkers Prev. PubMed
  4. Tai TS, et al. 2013. J. Immunol. 190:428. PubMed.
Product Citations
  1. Tai T, et al. 2013. J Immunol. 190:428. PubMed
  2. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  3. Jamroziak K, et al. 2009. Cancer Epidemiol Biomarkers Prev. 1.40625. PubMed
RRID
AB_314995 (BioLegend Cat. No. 313402)

Antigen Details

Structure
Protein tyrosine kinase, contains two SH2 domains, 70 kD
Distribution

T cells, NK cells, B-cell chronic lymphocytic leukemia (B-CLL)

Function
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP-70 causes selective T cell defect
Ligand/Receptor
Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc
Modification
Multiple phosphorylation sites (Tyr319 phosphorylation essential for full activity)
Cell Type
Leukemia, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1992. Cell 71:649.
2. Chan AC, et al. 1994. Science 264:1559.
3. Arpaia E, et al. 1994. Cell 76:947.

Gene ID
22637 View all products for this Gene ID 7353 View all products for this Gene ID
UniProt
View information about ZAP-70 on UniProt.org
Go To Top Version: 3    Revision Date: 08/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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