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Purified anti-human MRP1 (ABCC1) Antibody

Pricing & Availability
Clone
QCRL-3 (See other available formats)
Regulatory Status
RUO
Other Names
ABC29, ABCC, GS-X, MRP, Multidrug resistance-associated protein 1, LTC4 transporter, leukotriene C(4) transporter
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
QCRL-3_Pure_MRP1_ABCC1_Antibody_121815
H69AR cells were treated with the True-Nuclear™ Transcription Factor Buffer Set and then intracellularly stained with purified MRP1 (clone QCRL-3) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • QCRL-3_Pure_MRP1_ABCC1_Antibody_121815
    H69AR cells were treated with the True-Nuclear™ Transcription Factor Buffer Set and then intracellularly stained with purified MRP1 (clone QCRL-3) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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370302 100 µg $129
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Description

MRP1 is a 190 kD integral membrane phosphoglycoprotein. It is a member of the human ATP-binding cassette transporter multidrug resistance protein1 (MRP1), encoded by ABCC1. MRP1 is expressed in most normal tissues, with higher levels in the testes, lung, skin, small intestine, colon, kidney, placenta, peripheral blood mononuclear cells, and skeletal muscle. ABCC1 encodes plasma membrane proteins that have important physiological transport functions and mediate a variety of xenobiotics against their concentration gradients. MRP1 plays an important role in the extrusion of drugs from the cell and their over expression can be a cause of failure of anticancer and antimicrobial chemotherapy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
QCRL-3 was raised against membranes prepared from the human small cell lung cancer cell line, H69AR, which highly over expresses MRP1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.

Application Notes

QCRL-3 detects a discontinuous conformation-dependent epitope wholly contained in NBD1 of MRP1. The epitope for QCRL-3 has been mapped to aa 647-932. QCRL-3 is reported to be an effective and specific inhibitor of MRP1 activity when used in transport assays with inside-out membrane vesicles. QCRL-1, QCRL-2, QCRL-3, and QCRL-4 each bind distinct non-overlapping epitopes.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

RRID
AB_2566666 (BioLegend Cat. No. 370302)

Antigen Details

Structure
190 kD integral membrane phosphoglycoprotein.
Distribution

Testes, lung, skin, small intestine, colon, kidney, placenta, peripheral blood mononuclear cells, and skeletal muscle. Cytoplasm / plasma membrane.

Function
Extrusion of drugs from the cell.
Biology Area
Immunology
Antigen References

1. Cole SP, et al. 2014. Annu. Rev. Pharmacol. Toxicol. 54:95.
2. Hipfner DR, et al. 1999. J. Biol. Chem. 274(22):15420.
3. Filipits M, et al. 1999. Anticancer Res. 6:5043.
4. Wright SR, et al. 1998. Clin. Cancer Res. 4:2279.
5. Hipfner DR, et al. 1998. Br. J. Cancer 78(9):1134.
6. Filipits M, et al. 1997. Leukemia 11(7):1073.
7. Loe DW, et al. 1997. Mol. Pharmacol. 6:1034-41.
8. Hipfner DR, et al. 1996. Cancer Res. 56:3307.
9. Filipits M, et al. 1996. Clin. Cancer Res. 2(7):1231.
10. Almquist KC, et al. 1995. Cancer Res. 55(1):102.

Gene ID
4363 View all products for this Gene ID
UniProt
View information about MRP1 on UniProt.org
Go To Top Version: 1    Revision Date: 01/05/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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