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Purified anti-human PCSK9 Antibody

Pricing & Availability
Clone
M1118E3 (See other available formats)
Regulatory Status
RUO
Other Names
PCSK9, PC9, NARC1, Neural Apoptosis Regulated Convertase 1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
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M1118E3_Pure_PCSK9_Antibody_121718
  • M1118E3_Pure_PCSK9_Antibody_121718
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537502 500 µg $581
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Description

Proprotein convertase subtilisin/kexin 9 (PCSK9) belongs to the proteinase K subfamily of subtilases and is expressed as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. It is a 692 amino acid (aa) glycoprotein with an overall domain structure similar to other proprotein convertase members. It is comprised of a signal peptide (aa 1–30), a pro segment (aa 31–152), a catalytic domain (aa 153–407), a hinge region (aa 408–452), and a C-terminal Cys-His-rich domain (aa 453–692).

The biological roles of PCSK9 are closely related to its binding partners, low-density lipoprotein (LDL) and LDL receptor (LDLR). At the cell surface, secreted PCSK9 binds at a neutral pH to the EGF-A-like repeat of the LDLR via its catalytic domain. The binding of PCSK9 to LDLR enhances the degradation of the LDLR in endosomes/lysosomes, which results in increased circulating LDL-cholesterol (LDL-C). Thus, PCSK9 has gained attention as a pharmacological target for cardiovascular diseases and atherosclerosis. It also binds to the Apoliprotein B (ApoB) in LDL, although its role in this complex is not clearly understood. Due to the interaction of PCSK9 with LDLR, the levels of free PCSK9 or its complexed forms are important indicators for measuring risk of cardiovascular diseases or response to a therapy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of PCSK9 recombinant protein ranging from 58.6 to 3750 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified M1118E3 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5250 antibody (Cat. No. 525004) as the detection antibody and Recombinant Human PCSK9 (ELISA Std.) (Cat. No. 592509)

RRID
AB_2783350 (BioLegend Cat. No. 537502)

Antigen Details

Structure
Monomer
Distribution

Hepatocytes, neuro-epithelioma, colon carcinoma, Schwann cells

Function
PCSK9 regulates LDLR, VLDLR, APOER, and APOER2 levels, and, therefore LDLc level. PCSK9 is regulated by SREBP-2
Interaction
Hepatocytes
Ligand/Receptor
LDLR, VLDLR, APOB, LRP1, LRP8
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Enzymes and Regulators
Antigen References
  1. Seidah NG, et al. 2003. Proc Natl Acad Sci USA. 100:928.
  2. Naureckiene S, et al. 2003. Arch Biochem Biophys. 420:55.
  3. Costet P, et al. 2008. Trends Biochem Sci. 33:426.
  4. Tibolla G, et al. 2011. Nutr Metab Cardiovasc. Dis. 21:835.
Gene ID
255738 View all products for this Gene ID
UniProt
View information about PCSK9 on UniProt.org
Go To Top Version: 1    Revision Date: 12/17/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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  • Purified anti-human PCSK9

    M1118E3_Pure_PCSK9_Antibody_121718
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