Purified anti-human Pro-Major Basic Protein 1 (ProMBP1) Antibody

Pricing & Availability
Clone
J175-7D4 (See other available formats)
Regulatory Status
RUO
Other Names
ProMBP1
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_J175-7D4_A647_012110
Human peripheral blood lymphocytes surface stained with CD16 FITC, then intracellular stained with proMBP-1, clone J175-7D4, Alexa Fluor® 647
  • A_J175-7D4_A647_012110
    Human peripheral blood lymphocytes surface stained with CD16 FITC, then intracellular stained with proMBP-1, clone J175-7D4, Alexa Fluor® 647
  • B_J175-7D4_A647_012110
    Human peripheral blood lymphocytes surface stained with CCR3 PE, then intracellular stained with proMBP-1, clone J175-7D4, Alexa Fluor® 647
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346802 100 µg $265
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Description

Pro-form of Major Basic Protein 1 (ProMBP1) is composed of a strongly acidic 90-residue propart and a highly basic mature portion of 117 residues. It is an extensively and very heterogeneously glycosylated proteoglycan. ProMBP1 is processed to 14 kD MBP1 by convertase during maturation and localized in cell granule core. The mature MBP1 is a cytotoxic protein. The mAb J175-7D4 binds ProMBP1 and intermediate ProMBP1, but not mature MBP1. It  specifically stain basophils, does not stain eosinophils, granulocytes, and mast cells and can be used as a marker for human basophils.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant pro-form of human major basic protein 1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
IP, IHC-P - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections

Application References

(PubMed link indicates BioLegend citation)
  1. Plager D, et al. 2006. J. Allergy Clin. Immunol. 117:626
Product Citations
  1. Nojima T, et al. 2021. ORL J Otorhinolaryngol Relat Spec. 83:286. PubMed
  2. Zhao D, et al. 2022. J Transl Med. 20:386. PubMed
  3. Monte L, et al. 2016. Cancer Res . 76: 1792-1803. PubMed
RRID
AB_2099170 (BioLegend Cat. No. 346802)

Antigen Details

Structure
36-90kD heterogeneously glycosylated proteoglycan
Distribution

basophils

Cell Type
Basophils
Biology Area
Immunology, Innate Immunity
Gene ID
4153 View all products for this Gene ID
UniProt
View information about ProMBP1 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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