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- Clone
- TRA-2-54/2J (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Liver/Bone/Kidney Alkaline Phosphatase
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human teratocarcinoma cell line NCCIT cells were stained with purified TRA-2-54 (clone TRA-2-54/2J) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 358802 | 100 µg | $335 | ||||
TRA-2-54, also known as Liver/Bone/Kidney Alkaline Phosphatase (ALP or AP), is a tissue non-specific isoform of alkaline phosphatase-related antigen. This antigen is highly expressed on undifferentiated human embryonal carcinoma (EC) and embryonic stem (ES) cells, and is also expressed by liver, bone and kidney cells. It has been reported that the expression level of TRA-2-54 is decreased upon differentiation; therefore measurement of TRA-2-54 expression can be used to monitor the differentiation status of pluripotent stem cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human embryonal carcinoma cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for relevant formats) include: immunohistochemistry1 and immunofluorescence1.
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Application References
(PubMed link indicates BioLegend citation) -
- Zou XY, et al. 2012. Stem Cell Res. Ther. 3: 43. (IHC, IF)
- RRID
-
AB_2562264 (BioLegend Cat. No. 358802)
Antigen Details
- Distribution
-
Undifferentiated embryonic stem (ES) cells, embryonal carcinoma (EC) cells, most EC cell lines, bone, liver, kidney
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Andrews PW, et al. 1996. Int. J. Cancer. 66:806.
2. Yan X, et al. 2010. Stem Cells Dev. 19:469.
3. Zou XY, et al. 2012. Stem Cell Res Ther. 3:43. - Gene ID
- 249 View all products for this Gene ID
- UniProt
- View information about TRA-2-54 on UniProt.org
Other Formats
View All TRA-2-54 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human TRA-2-54 | TRA-2-54/2J | FC,ICC |
| PE anti-human TRA-2-54 | TRA-2-54/2J | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TRA-2-54
Human teratocarcinoma cell line NCCIT cells were stained wit... -
PE anti-human TRA-2-54
Human teratocarcinoma cell line NCCIT cells were stained wit...
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