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Purified anti-human VEGF Antibody

Pricing & Availability
Clone
A15136H (See other available formats)
Regulatory Status
RUO
Other Names
VEGFA, VEGF-A, VPF, MVCD1, vascular endothelial growth factor
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
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  • A15136H_Pure_VEGF_Antibody_121218.png
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537002 500 µg $581
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Description

VEGF (also known as VEGF-A) is a signaling protein with strong vascular permeability activity that stimulates the formation of new blood vessels during the processes of vasculogenesis and angiogenesis. It is a member of the vascular endothelial growth factor family, which in mammals is comprised of VEGF-A, VEGF-B, VEGF-C, VEGF-D, and PlGF (placental growth factor). There are multiple isoforms of VEGF that result from alternative splicing of mRNA. VEGF binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), through which VEGF exerts its mitogenic effects. VEGF is highly expressed in solid tumors of breast, lung, renal, colorectal, and hepatic origin. Additionally, it significantly contributes to ascites tumor formation. Beyond cancer, VEGF has also been associated with multiple pathogenic conditions including rheumatoid arthritis, age-related macular degeneration, Crow-Fukase syndrome, sclerosis and Alzheimer’s disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1 - 4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of VEGF recombinant protein ranging from 23.4 to 1,500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified A15136H antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5225 antibody (Cat. No. 522503) as the detection antibody and Recombinant Human VEGF (ELISA Std.) (Cat. No. 560309)

RRID
AB_2783345 (BioLegend Cat. No. 537002)

Antigen Details

Structure
Homodimer
Distribution

Widely expressed

Function
VEGFA is a key player in vasculogenesis, the formation of blood vessels from progenitor cells, as well as angiogenesis. The expression of the VEGFA gene is upregulated via hypoxia, estrogen, and NF-κB pathways. In addition VEGF is upregulated by PDGF-BB, P1GF, TGFβ1, IGF1, FGFs, HGF, TNFα, and IL-1. VEGFA induces proliferation and cell migration in endothelial cells and plays important roles during wound healing. Also, VEGF regulates hematopoietic stem cell survival.
Interaction
VEGFA interacts with vascular endothelial cells and monocytes/macrophages, which express VEGFR1. This interaction induces proliferation of endothelial cells and stimulates migration of monocytes/macrophages
Ligand/Receptor
Vascular endothelial growth factor (VEGF)-A binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1)
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Conn G, et al. 1990. Proc. Natl. Acad. Sci USA 87:1323.
  2. Gerber H, et al. 2002. Nature 417:954.
  3. Shibuya M. 2006. J. Biochem. Mol. Biol. 39:469.
  4. Shibuya M. 2008. BMB Rep. 41:278.
  5. Monaghan-Benson E, et al. 2010. The American Journal of Pathology 177:2091.
  6. Koch S and Claesson-Welsh L. 2012. Cold Spring Harb Perspect Med. 2:a006502.
Gene ID
7422 View all products for this Gene ID
UniProt
View information about VEGF on UniProt.org
Go To Top Version: 1    Revision Date: 12/11/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-human VEGF

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