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Purified anti-IGF2BP2 Antibody

Pricing & Availability
Clone
O93C8 (See other available formats)
Regulatory Status
RUO
Other Names
IMP2, Hepatocellular Carcinoma Autoantigen P62, VICKZ2, IGF-II mRNA binding protein, Insulin-like growth factor 2, P62
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O93C8_PURE_IGF2BP2_Antibody_1_110818
Total cell lysates (15 µg total protein) from A431, HaCat, HepG2, HeLa (reduced expression control) and U937(reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-IGF2BP2 Antibody, clone O93C8, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • O93C8_PURE_IGF2BP2_Antibody_1_110818
    Total cell lysates (15 µg total protein) from A431, HaCat, HepG2, HeLa (reduced expression control) and U937(reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-IGF2BP2 Antibody, clone O93C8, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • O93C8_PURE_IGF2BP2_Antibody_2_110818
    HeLa cells were stained with purified anti-IGF2BP2 (clone O93C8) antibody, followed by staining with Alexa Fluor® 594 conjugated goat anti-mouse IgG antibody (red). Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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679602 100 µg $335
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Description

IGF2BP2 belongs to the family of Insulin-like growth factor-II mRNA binding proteins (IMP1, IMP2, and IMP3). Members in this family contain four hnRNP K homology (KH) domains and two RNA recognition motifs (RRM) domains. They are involved in the RNA cytoplasmic localization, stability, and translation. Recent studies suggest that IGF2BP2 and IGF2BP1 bind to target mRNAs via all four KH domains. Studies showed that the expression of IGF2BP2 is associated with liposarcoma, liver cancer, and endometrial adenocarcinomas. Genome-wide association studies have also identified IGF2BP2 as one of the several factors for developing type 2 diabetes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human IGF2BP2 protein (NP_006539) produced in HEK 293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 0.1-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566241 (BioLegend Cat. No. 679602)

Antigen Details

Structure
Canonical sequence contains 599 amino acids with a predicted molecular weight of 66.1 kD. Five isoforms with a molecular weight ranging from 54.7 kD to 61.8 kD.
Distribution

Nucleus, cytoplasm, and the cytoskeleton. It is expressed in oocytes, granulosa cells, Leydig cells, spermatogonia, but is expressed weaker in the lymph nodes, lungs, liver, kidney, heart muscles, spleen, and testis.

Function
IGF2BP2 binds to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulates IGF2 translation. It also modulates the cytoplasmic trafficking, turnover, and translation of various target mRNAs.
Interaction
Interacts with HNRPD, IGF2BP1, IGF2BP3, ELAVL1, DHX9, and HNRNPU.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Wachter K, et al. 2013. Biol. Chem. 394:1077.
2. Zhao Y, et al. 2012. DNA Cell Biol. 31:713.
3. Bell JL, et al. 2013. Cell Mol. Life Sci. 15:2657.
4. Ng MC, et al. 2008. Diabetes 57:2226.
5. Scott LJ, et al. 2007. Science 316:1341.
6. Nielsen FC, et al. 2002. J. Cell Sci. 115:2087.
7. Nielsen J, et al. 1999. Mol. Cell Biol. 2:1262.

Gene ID
10644 View all products for this Gene ID
UniProt
View information about IGF2BP2 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 11/08/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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