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Purified anti-IGFBP-3 Antibody

Pricing & Availability
Clone
M6501G03 (See other available formats)
Regulatory Status
RUO
Other Names
Insulin-like growth factor binding protein 3, IGF-Binding Protein 3, Growth Hormone-Dependent Binding Protein, Binding Protein 29, IBP3, BP-53, IGFBP3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M6501G03_PURE_IGFBP-3_Antibody_WB_111715
Western blot analysis of recombinant human IGFBP-3. Anti-IGFBP-3 antibody (clone M6501G03) was used, followed by HRP anti-mouse IgG antibody.
  • M6501G03_PURE_IGFBP-3_Antibody_WB_111715
    Western blot analysis of recombinant human IGFBP-3. Anti-IGFBP-3 antibody (clone M6501G03) was used, followed by HRP anti-mouse IgG antibody.
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679702 100 µg $311
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Description

The insulin-like growth factor system includes two receptors (IGF-1R and IGF-2R), two ligands (IGF-1 and IGF-2), and seven binding proteins (IGFBP1-7). The binding proteins are produced in the liver and bind to the IGFs with different affinities, thus regulating the interaction of the IGFs with their receptors. Insulin-like growth factor binding protein-3 (IGFBP-3) is the major serum carrier protein for IGF proteins. IGFBP-3 sequesters IGF in a stable ternary complex with the acid-labile subunit (ALS). IGFBP-3 binds IGF-1 with high affinity and specificity and modulates IGF-1 action. IGFBP-3 undergoes limited enzymatic proteolysis to generate small N-terminal fragments. These proteolytic products lose their affinity for IGF-1 and IGF-2. Nevertheless, they preserve IGF independent activity. In that sense, a 16 kD fragment (1-95-IGFBP-3) and the full length molecule can potentiate apoptosis in colorectal epithelial cells, and this apoptotic process is associated with the inhibition of TNF-α-induced NF-κB activity. In addition, IGFBP-3 has been detected in the cell nucleus and interacts with 9-cis retinoic acid receptor-alpha (RXR-α), which is a nuclear receptor that is required for the apoptosis-inducing effects of IGFBP-3. IGFBP-3R physically interacts and activates caspase-8 and mediates IGFBP-3-induced apoptosis and tumor suppression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IGFBP-3, amino acids (Gly28-Glu291) (Accession# X64875) with an N-terminal Met, was expressed in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566243 (BioLegend Cat. No. 679702)

Antigen Details

Structure
291 amino acids with a predicted molecular weight of approximately 32 kD. The glycosylated form migrates at approximately 45-50 kD by SDS-PAGE.
Distribution

IGFBP-3 is expressed by most of the tissues and is present in plasma.

Function
Transports insulin like growth factors (IGFs) and protects them from rapid clearance and/or degradation as well as modulating IGFs bioavailability. IGFBP-3 induces apoptosis in vitro and suppresses tumors in vivo. IGFBP-3 is induced by growth hormones, TGF-β, vitamin D, retinoids, and anti-estrogens.
Interaction
Interacts with XLKD1, IGF2, IGF1, ALS, HN, and TMEM219.
Ligand/Receptor
IGFBP-3R (TMEM219).
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Antigen References

1. Cohen P, et al. 1992. J. Clin. Endocrinol. Metab. 75:1046.
2. Lee KW and Cohen P. 2002. J. Endocrinol. 175:33.
3. Yan X, et al. 2004. J. Biol. Chem. 279:53232.
4. Shahjee H, et al. 2008. Growth Horm. IGF Res. 18:188-97.
5. Berrigan D, et al. 2009. Growth Horm. IGF Res. 2:146.
6. Ingermann AR, et al. 2010. J. Biol. Chem. 285:30233.
7. Forbes BE, et al. 2012. Front. Endocrinol. 3:38.
8. Baxter RC, et al. 2014. Nat. Rev. Cancer 14:329.

Gene ID
3486 View all products for this Gene ID
UniProt
View information about IGFBP-3 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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