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Purified anti-IKKγ (NEMO) Antibody

Pricing & Availability
Clone
W16070A (See other available formats)
Regulatory Status
RUO
Other Names
NF-kappa-B essential modulator, FIP-3, IKKAP1, IKK-gamma, IKKG, IKBKG
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W16070A_PURE_IKKy_NEMO_Antibody_1_WB_011617
Total cell lysates (15 µg protein) were resolved by 4-12% Bis-tris gel electrophoresis and probed with 1 µg/mL purified anti-IKKγ (NEMO) antibody (clone W16070A) or competitors clone at the recommended concentration of the manufacturer. Proteins were visualized using the appropriate secondary antibodies. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control.
  • 1_W16070A_PURE_IKKy_NEMO_Antibody_1_WB_011617
    Total cell lysates (15 µg protein) were resolved by 4-12% Bis-tris gel electrophoresis and probed with 1 µg/mL purified anti-IKKγ (NEMO) antibody (clone W16070A) or competitors clone at the recommended concentration of the manufacturer. Proteins were visualized using the appropriate secondary antibodies. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control.
  • 2_W16070A_PURE_IKKgamma_NEMO_IF_092517
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for one hour. Then the cells were intracellularly stained with 2 µg/mL of purified anti-IKKγ (NEMO) antibody (clone W16070A) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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694101 25 µg $101
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694102 100 µg $270
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Description

NF-kB represents a family of transcription factors that are normally inactive in the cytoplasm by binding inhibitory molecules of the IkB proteins. In response to various stimuli such as: inflammatory cytokines, bacterial or viral products, or various types of stress, the IkB proteins become phosphorylated by Iκ B kinase (IKK) complex. This leads to the polyubiquitination and destruction by the proteasome. Consequently, free NF-kB enters the nucleus and activates transcription of a variety of genes involved in immune and inflammatory response, cell adhesion, growth control, and inhibition of apoptosis. The IKK complex consists of the regulatory subunit IKKγ/NEMO (NF-κ B Essential Modifier) and two catalytic subunits (IKKα and IKKβ). IKKγ is an adaptor protein involved in activation of the IKK kinases and has been shown to be essential for the canonical and stress-induced NF-κ B pathway. Recently, Medunjanin S, et al reported that GSK-3β is critically important for ordered NF-κB signaling through modulation of IKKγ phosphorylation.

IKKγ deficiency in mice resulted in a lack of detectable NF-κ B DNA-binding activity and a lethal embryonic phenotype because of severe liver damage due to massive apoptosis. Two distinct X-linked human diseases, incontinentia pigmenti (IP) and anhidrotic ectodermal dysplasia associated with immunodeficiency (EDA-ID), have been linked to IKKγ dysfunction; indicating the critical roles of IKKγ in human development, skin homeostasis, and innate/acquired immunity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human IKKγ (240-419 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W16070A does not cross-react with mouse (in-house tested).

RRID
AB_2650723 (BioLegend Cat. No. 694101)
AB_2650723 (BioLegend Cat. No. 694102)

Antigen Details

Structure
419 amino acids, and the predicted molecular weight is 48 kD.
Distribution

Cytoplasm, nucleus.

Function
Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B, thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor.
Interaction
Component of the IKK core complex consisting of CHUK, IKBKB and IKBKG. Interacts with COPS3, CYLD, NALP2, TRPC4AP and LRDD. Interacts with ATM, TRAF6, IKBKE, TANK, ZFAND5, RIPK2, TNIP1 and TNFAIP3, polyubiquitin, USP10, ZC3H12A.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Israël A. 2010. Cold Spring Harb. Perspect. Biol. 2:a000158
2. Rothwarf DM, et al. 1998. Nature. 395:297.
3. Medunjanin S, et al. 2016. Sci. Rep. 6:38553.
4. Rudolph D, et al. 2000. Genes. Dev. 14:854
5. Smahi A, et al. 2000. Nature. 405: 466
6. Fusco F, et al. 2008. Hum. Mutat. 29: 595

Gene ID
8517 View all products for this Gene ID
UniProt
View information about IKKgamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/16/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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