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Purified anti-IL-27/IL-35 EBI3 Antibody

Pricing & Availability
Clone
A15058A (See other available formats)
Regulatory Status
RUO
Other Names
Epstein-Barr Virus Induced 3, EBV-Induced Gene 3 Protein, IL-27 Subunit Beta, IL-27B, IL-35 subunit
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15058A_PURE_IL27_IL35_Antibody_1_WB_031416
Western blot analysis of 50 ng of Recombinant Human IL-27 (Lane 1), Recombinant Mouse IL-27 (Lane 2), and Recombinant Human IL-35-Fc fusion protein (ELISA Std.) (Lane 3). Mouse anti-human IL-27/IL-35 EBI3 antibody (clone A15058A) was used, followed by HRP Goat anti-mouse IgG (minimal x-reactivity) antibody.
  • A15058A_PURE_IL27_IL35_Antibody_1_WB_031416
    Western blot analysis of 50 ng of Recombinant Human IL-27 (Lane 1), Recombinant Mouse IL-27 (Lane 2), and Recombinant Human IL-35-Fc fusion protein (ELISA Std.) (Lane 3). Mouse anti-human IL-27/IL-35 EBI3 antibody (clone A15058A) was used, followed by HRP Goat anti-mouse IgG (minimal x-reactivity) antibody.
  • A15058A_PURE_IL27_IL35_Antibody_2_WB_031416
    Western blot analysis of cell lysates from THP-1 untreated (lane 1), THP-1 PMA 100 ng/ml for three days followed by LPS 1 μg/ml for one day (Lane 2), Raw264.7 untreated (Lane 3), and Raw264.7 starved overnight and treated with LPS 1μg/ml for one day (lane 4). Mouse anti-human IL-27/IL-35 EBI3 antibody (clone A15058A) was used, followed by HRP Goat anti-mouse IgG (minimal x-reactivity) antibody. Purified anti-SHIP-1 antibody was used as a loading control.
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684602 100 µg $265
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Description

EBI3 is an IL-12 p40-related protein that can be heterodimerized with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells following stimulation through TLR. EBI3 can regulate T cell differentiation and proliferation. IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 differentiation, and impact B cell differentiation and immunoglobulin production. In addition to this, both IL-27 and IL-35 increase cytolytic activity of CD8+ T cells. IL-35 is also involved in tumorigenesis and is a promising biomarker for assessing tumor progression and prognosis in a clinical setting.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-35-Fc fusion protein expressed in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone A15058A does not react with mouse EBI3.

RRID
AB_2572169 (BioLegend Cat. No. 684602)

Antigen Details

Structure
229 amino acids with a predicted molecular weight of approximately 25 kD.
Distribution
B cells, monocytes, and dendritic cells.
Function
Pro- and anti-inflammatory.
Interaction
IL-27 p28 subunit and IL-35 p35 subunit.
Ligand/Receptor
WSX-1(TCCR) and gp130.
Cell Type
B cells, Monocytes, Dendritic cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pflanz S, et al. 2002. Immunity. 16:779.
2. Takeda A, et al. 2003. J. Immunol. 170:4886.
3. Pflanz S, et al. 2004. J. Immunol. 172:2225.
4. Li MF, et al. 2013. Dev. Comp. Immunol. 41:514.
5. Zeng JC, et al. 2013. Int. J. Clin. Exp. Pathol. 6:1806.
6. Ouyang H, et al. 2014. Am. J. Med. Sci. 348:156.

Gene ID
10148 View all products for this Gene ID
Go To Top Version: 1    Revision Date: 03/18/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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