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Purified anti-IL-36α Antibody

Pricing & Availability
Clone
O91D11 (See other available formats)
Regulatory Status
RUO
Other Names
IL-36 alpha, IL-1F6, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon
Isotype
Mouse IgG1, κ
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Product Citations
publications
O91D11_Purified_IL-36alpha_Antibody_WB_043015
Recombinant human IL-36α (50 ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IL-36α antibody (clone O91D11) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • O91D11_Purified_IL-36alpha_Antibody_WB_043015
    Recombinant human IL-36α (50 ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IL-36α antibody (clone O91D11) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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676402 100 µg $229
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Description

IL-36α is one of the IL-36 cytokines that are part of the IL-1 family. Like other IL-1 family members, IL-36α requires N-terminal processing to gain more potent bioactivity. Currently, the proteases responsible for processing IL-36α are unknown. IL-36α signals through IL-36R/IL-1RAcP, which results in MAPK, Erk1/2, and JNK activation. IL-36α is implicated in skin homeostasis, and it is overexpressed in psoriatic lesional skin. Transgenic mice overexpressing IL-36α in skin have an inflammatory skin condition, which shows some characteristics of human psoriasis, including thickened scaly skin, acanthosis, hyperkeratosis, and dermis infiltration. EGF regulates the expression of IL-36α in the skin. IL-36α can also be detected in adipose tissue. IL-36α reduces adipocyte differentiation and also induces inflammatory gene expression in mature adipocytes. In the lungs, the expression of IL-36α is increased in response to inflammatory stimuli. Intratracheal instillation of recombinant mouse IL-36α induces CXCL1 and CXCL2 expression along with neutrophil influx in the lungs. IL-36α, IL-36β, and IL-36γ induce in vitro expression of the RN of multiple cytokines (IL-6, IL-12 p40, CXCL1, CCL1, IL-12 p35, IL-1β, IL-23 p19, GM-CSF, CXCL10, TNF-α, CCL3, VCAM-1, and ICAM-1) in mouse bone marrow-derived dendritic cells and CD4 T cells obtained from normal mice. IL-36α expression is elevated in chronic kidney disease and in rheumatoid arthritis synovium, and its decreased expression correlates with a poor prognosis in hepatocellular carcinoma.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human IL-36α (NP_055255) produced in HEK 293T cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565642 (BioLegend Cat. No. 676402)

Antigen Details

Structure
Full length IL-36α contains 167 amino acids with a predicted molecular weight of 17.7 kD. Proccessing at the N-terminal of IL-36α results in higher potency.
Distribution

Monocytes, T and B cells, spleen, bone marrow, tonsils, lymph nodes, skin, lung, fetal brain, stomach, and adipose tissue.

Function
IL-36α participates in inflammatory reactions, induces multiple cytokines in vivo and in vitro, and is involved in skin homeostasis and reduction of adipocyte differentiation. IL-17 and TNF induce IL-36α expression in keratinocytes, and IL-22 synergizes this induction. EGF also regulates the expression of IL-36α in the skin.
Interaction
Macrophages, adipocytes, lung fibroblasts, bone marrow dendritic cells, and T cells.
Ligand/Receptor
IL36R/IL-RAcP.
Cell Type
B cells, Dendritic cells, Monocytes, T cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Gresnigt MS and van de Veerdonk FL. 2013. Semin  Immunol. 6:458.
2. Towne JE and Sims JE. 2012. Curr. Opin. Pharmacol. 12:486.
3. Vigne S, et al. 2011. Blood 118:5813.
4. Towne JE, et al. 2011. J. Biol. Chem. 286:42594.
5. Chustz RT, et al. 2011. J. Respir. Cell Mol. Biol. 45:145.
6. Vos JB, et al. 2005. Physiol. Genomics 21:324.
7. Pan QZ, et al. 2013. Cancer Immunol. Immunother. 62:1675.

Gene ID
27179 View all products for this Gene ID
UniProt
View information about IL-36alpha on UniProt.org

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Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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