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Purified anti-IREB2 Antibody

Pricing & Availability
Clone
W18260A (See other available formats)
Regulatory Status
RUO
Other Names
Iron-responsive element-binding protein 2 (IRE-BP 2, IREB2), Iron Regulatory Protein 2 (IRP2)
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
A. W18260A_PURE_IREB2_Antibody_110222
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-IREB2 (clone W18260A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • A. W18260A_PURE_IREB2_Antibody_110222
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-IREB2 (clone W18260A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • B. W18260A_PURE_IREB2_Antibody_2_112922
    Whole cell extracts (250 µg total protein) prepared from HEK293T cells treated with 100 µM DFO overnight were immunoprecipitated overnight with 2.0 µg of purified rat IgG2a, κ isotype control (Cat. No. 400502) or purified anti-IREB2 (clone W18260A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with HRP Donkey anti-rabbit IgG (clone Poly4064) (Cat. No. 406401) against IREB2. Lane M: Molecular weight marker
  • C. W18260A_PURE_IREB2_Antibody_3_112922
    IHC staining of purified anti-IREB2 (clone W18260A) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated without (panel A) and with (panel B) 5 μg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • D. W18260A_PURE_IREB2_WB_042224
    Whole cell extracts (15 µg total protein) from HEK293T cell lines treated without (-) and with (+) DFO were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-IREB2 (clone W18260A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664803) was used as a loading control at a 1:50000 dilution. Western-Ready ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
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608951 25 µg $155
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608952 100 µg $400
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Description

IREB2 is an iron-responsive protein that regulates a cell’s levels of iron, a key micronutrient. In low-iron situations, IREB2 is free to bind to iron-responsive elements in mRNA molecules, stabilizing transferrin mRNA to increase expression of the iron uptake receptor, while blocking the translation of ferritin mRNA, a protein which sequesters excess iron molecules. However, when sufficient iron levels are present, IREB2 undergoes a conformation change to lose its mRNA binding ability, resulting in the degradation of transferrin mRNA while releasing the translation block of ferritin. Variants in the gene encoding IRP2 have been associated with lung cancer and chronic obstructive pulmonary disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human IREB2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product was tested for use in ICC using PFA-Triton-X, PFA-MeOH, and MeOH only fix-perm methods, and ICFC using the Cyto-fix method, but did not meet passing criteria. 

This product was tested for mouse reactivity in WB and ICFC but did not meet passing criteria. 

RRID
AB_2927977 (BioLegend Cat. No. 608951)
AB_2927977 (BioLegend Cat. No. 608952)

Antigen Details

Structure
IREB2 is a 953 amino-acid protein with a predicted molecular weight of 105 kD.
Distribution

IREB2 is expressed primarily in the cytoplasm

Function
IREB2 is an iron sensor
Interaction
IREB binds to iron-responsive elements in the mRNA sequences of mRNAs such as ferritin and transferrin
Ligand/Receptor
IREB2 binds iron cations
Biology Area
Chromatin Remodeling/Epigenetics
Antigen References
  1. Papanikolaou G, et al. 2017. IUBMB Life. 69:399-413.
  2. Gozzelino R, et. al. 2016. Int J Mol. Sci. 17:130.
Gene ID
3658 View all products for this Gene ID
UniProt
View information about IREB2 on UniProt.org
Go To Top Version: 1    Revision Date: 11/02/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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