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Purified anti-IRF4 Antibody

Pricing & Availability
Clone
IRF4.3E4 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 4
Isotype
Rat IgG1, κ
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Product Citations
publications
1_IRF43E4_Purified_IRF4_Purified_Antibody_WB_050918
Total lysates (15 µg protein) from HeLa (lane 1, negative control) and Ramos (lane 2) were resolved by electrophoresis (4-12% Bis-Tris midi gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) Purified anti-IRF4 Antibody, clone IRF4.3E4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-rat-IgG secondary antibody conjugated to HRP for the anti-IRF4 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 1_IRF43E4_Purified_IRF4_Purified_Antibody_WB_050918
    Total lysates (15 µg protein) from HeLa (lane 1, negative control) and Ramos (lane 2) were resolved by electrophoresis (4-12% Bis-Tris midi gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) Purified anti-IRF4 Antibody, clone IRF4.3E4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-rat-IgG secondary antibody conjugated to HRP for the anti-IRF4 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 2_IRF43E4_GoChIP_IRF4_Antibody_1_020618.png
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from U266 cells treated with IFNα2 with either A) 1:200 dilution of Go-ChIP-Grade™ Purified anti-IRF4 (Clone IRF4.3E4), or B) equal amount of Purified Rat IgG1, κ Isotype Control Antibody (Cat. No. 400401). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human SUB1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
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646402 100 µg $265
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Description

The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
GST fusion protein containing C-terminal of murine IRF4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Verified
ICFC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg/ml. For ChIP applications, the suggested dilution is is 1:50-1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Zheng Y, et al. 2009. Nature 458:351
  2. Yin SY, et al. 2011. Exp Cell Res. 317:2210. PubMed
  3. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  4. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  2. Xiong E, et al. 2023. Front Immunol. 13:1083119. PubMed
  3. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  4. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  5. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  6. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  7. Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed
  8. Trudu M, et al. 2022. Front Oncol. 12:968208. PubMed
  9. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  10. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  11. Yin S, et al. 2011. Exp Cell Res. 317:2210. PubMed
  12. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID
AB_2280462 (BioLegend Cat. No. 646402)

Antigen Details

Structure
Molecular weight is approximately 51 kD.
Distribution

IRF4 is reported to be expressed exclusively in the immune system.

Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lu R. 2008. Trends Immunol 29:487.

Gene ID
3662 View all products for this Gene ID
UniProt
View information about IRF4 on UniProt.org
Go To Top Version: 4    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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