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Purified anti-KCNA5 Antibody (Previously Covance catalog# MMS-5035)

Pricing & Availability
Clone
2D10.D6 (See other available formats)
Regulatory Status
RUO
Other Names
HK2, HCK1, PCN1, ATFB7, HPCN1, KV1.5, potassium voltage-gated channel subfamily A member 5, voltage-gated potassium channel protein Kv1.5
Previously
Covance Catalog# MMS-5035
Isotype
Mouse IgG1, κ
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Product Citations
publications
2D10dotD6_Purified_KCNA5_Antibody_1_101518
IHC staining of purified anti-KCNA5 antibody (clone 2D10.D6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2D10dotD6_Purified_KCNA5_Antibody_1_101518
    IHC staining of purified anti-KCNA5 antibody (clone 2D10.D6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2D10dotD6_Purified_KCNA5_Antibody_2_101518
    Western blot of purified anti-KCNA5 antibody (clone 2D10.D6). Lane 1: Molecular weight marker; Lane 2: 20 µg of U87 cell lysate; Lane 3: 20 µg of U251 cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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814901 100 µL $275
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Description

KCNA5 is a potassium voltage-gated ion channel with 6 transmembrane spanning domains, also known as Kv1.5, belonging to the shaker-related subfamily of potassium channels. It forms tetrameric potassium selective channels that open or close based on the voltage difference across the membrane. Potassium channels contribute to a diverse set of functions depending on the cell type and localization, some of which include: regulating neurotransmitter release, heart rate, insulin secretion, smooth muscle contraction, neuronal excitability, and much more.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence corresponding to amino acids 53-64 of the human KCNA5 protein conjugated to KLH.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P).

Clone is less reactive in human samples in IHC-P applications.

RRID
AB_2564788 (BioLegend Cat. No. 814901)

Antigen Details

Structure
Expected MW: 67 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
3741 View all products for this Gene ID
UniProt
View information about KCNA5 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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