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Purified anti-KDM1A Antibody

Pricing & Availability
Clone
W20070A (See other available formats)
Regulatory Status
RUO
Other Names
Lysine-specific histone demethylase 1A, KDM1A, BRAF35-HDAC complex protein, BHC110, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A, AOF2, KDM1, KIAA0601, LSD1, EC:1.14.99.66
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W20070A_PURE_KDM1A_Antibody_WB_020623
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-KDM1A (clone W20070A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL substrate premium kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W20070A_PURE_KDM1A_Antibody_WB_020623
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-KDM1A (clone W20070A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL substrate premium kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W20070A_PURE_KDM1A_Antibody_IP_020623
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype control (Cat. No. 400502) or purified anti-KDM1A (clone W20070A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of KDM1A. Lane M: Molecular weight marker
  • c. W20070A_PURE_KDM1A_Antibody_IHC-P_020623
    IHC staining of purified anti-KDM1A (clone W20070A) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using 1X Tris-EDTA buffer (10mM Tris, 1mM EDTA, pH 9.0), the tissue was incubated without (panel A) or with 1 µg/mL of anti-KDM1A (panel B), overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (red) (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and images were captured with a 60X objective. Scale bar: 50 μm
  • d. W20070A_PURE_KDM1A_Antibody_ICC_updated_021723
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 1.5 µg/mL of either purified rat IgG2a, κ isotype control (Cat. No. 400502) (panel A) or purified anti-KDM1A (clone W20070A) (panel C) overnight at 4°C followed by incubation with of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) (panel B shows merged image of panel A and C) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel D- DAPI alone), and the image was captured with a 60X objective. Scale bar: 25 µm
Cat # Size Price Quantity Check Availability Save
610851 25 µg $120
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610852 100 µg $295
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Description

KDM1A (or LSD1) belongs to the flavin adenine dinucleotide (FAD)-dependent demethylase family and has enzymatic activity on de-methylation lysine-4 and lysine-9 of Histone H3. During early stage embryonic development, KDM1A regulates differentiation and development through corepressor of RE1-silencing transcription factor (CoREST) complex and histone deacetylases (HDACs). KDM1A participates in nuclear hormone signaling by interacting with androgen receptor (AR) and estrogen receptor (ER) of the receptor-expressing tissues in the regulation of cancer progression and metastasis. In addition to histone H3, KDM1A demethylates lysine residues of p53, E2F1, or DNMT1 which modulates apoptosis and cell proliferation. Dysfunction of KDM1A is found in acute myeloid leukemia (AML), T cell acute lymphoid leukemia (T‑ALL), and several malignancies, implying it is a potential epigenetic biomarker and drug target.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human KDM1A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W20070A does not cross-react with mouse in western blot.

For immunocytochemistry (ICC), the suggested fixation/permeabilization method is either 4% paraformaldehyde/0.5% Triton X-100 or paraformaldehyde followed by ice-cold methanol.

For immunohistochemistry, the suggested antigen retrieval buffer is Tris-EDTA pH 9.0.

Additional Product Notes

ICC: suggest PFA+TX and PFA-Methanol

RRID
AB_2936733 (BioLegend Cat. No. 610851)
AB_2936733 (BioLegend Cat. No. 610852)

Antigen Details

Structure
KDM1A is an 852 amino-acid protein with a predicted molecular weight of 92.9 kD, and alternative splicing isoform is 876 amino-acid at 95.2 kD.
Distribution

Ubiquitous/Nuclear  

Function
An epigenetic demethylase which removes methylations of histone at lysine-4 (H3K4me) and lysine-9 (H3K9me).
Interaction
Histone H3, P53, HDAC1, DNMT, CoREST, E2F1, Androgen receptor, and Estrogen receptor.
Ligand/Receptor
Flavin adenine dinucleotide (FAD)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Immunology, Neuroscience, Signal Transduction, Stem Cells, Ubiquitin/Protein Degradation
Molecular Family
Nuclear Markers, Protein Modifiers
Antigen References
  1. Huang J, et al. 2007. Nature. 449:105-8.
  2. Wang J, et al. 2009. Nat Genet. 41:125-9.
  3. Kontaki H, et al. 2010. Mol Cell. 39:152-60.
  4. Foster CT, et al. 2010. Mol Cell Biol. 30:4851-63.
  5. Vu LP, et al. 2013. Int J Hematol. 97:198-209.
Gene ID
23028 View all products for this Gene ID
UniProt
View information about KDM1A on UniProt.org
Go To Top Version: 1    Revision Date: 02/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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