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Purified anti-LC3 Antibody

Pricing & Availability
Clone
A15143K (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated proteins 1A/1B light chain 3B, MAP1A/MAP1B LC3 B, MAP1A/1B light chain 3 B, MAP1 light chain 3-like protein 2, autophagy-related ubiquitin-like modifier LC3 B
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15143K_PURE_LC3_Antibody_1_021218
IHC staining of purified anti-LC3 antibody (clone A15143K) on formalin-fixed paraffin-embedded human and rat brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissues were incubated with the primary antibody at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • A15143K_PURE_LC3_Antibody_1_021218
    IHC staining of purified anti-LC3 antibody (clone A15143K) on formalin-fixed paraffin-embedded human and rat brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissues were incubated with the primary antibody at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • A15143K_PURE_LC3_Antibody_2_021218
    ICC staining of purified anti-LC3 antibody (clone A15143K) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/mL of the primary antibody for three hours at room temperature, followed by 1 µg /mL of Alexa Fluor® 488 goat anti-mouse IgG antibody (Cat. No. 405319) (green). Cells were then incubated with 1 µg/mL of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) antibody (Cat. No. 801210) (pink) overnight at 4°C. Nuclei were stained with Hoechst 33342 (blue) at 5 µg/mL for 10 minutes at room temperature. Images were captured with a 60X objective.
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848801 25 µg $112
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848802 100 µg $276
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Description

Microtubule-associated protein light chain 3, commonly known as LC3, is a central protein in the autophagy pathway and functions in autophagosome biogenesis. LC3 is a member of the ATG8 protein family and expressed as three isoforms: LC3A (MAP1LC3A), LC3B (MAP1LC3B), and LC3C (MAP1LC3C). Newly synthesized LC3 is hydrolyzed by the cysteine protease ATG4B to produce the active cytosolic form termed LC3I. Through a series of reactions involving other ATGs, LC3I becomes attached to phosphatidylethanolamine. This lipid modified form is termed LC3II, and is involved in autophagosomes membrane expansion and fusion events. LC3 is widely used as a marker for autophagosomes. Defects in autophagy machinery have been associated with multiples diseases including neurodegenerative disorders.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A synthetic peptide corresponding to N-terminal region of human LC3B protein (between amino acid residues 1-50) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC - Verified 
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 1.0 µg/mL is suggested  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody cross-reacts with LC3A and LC3B isoforms.

Additional reported applications (for the relevant formats) include: western blotting

Product Citations
  1. Jiang W, et al. 2022. Front Physiol. 13:949737. PubMed
  2. Al–Ramahi I, et al. 2018. Cell Syst. 7:28. PubMed
  3. Stykel MG, et al. 2021. Cell Reports. 35(6):109099. PubMed
RRID
AB_2650713 (BioLegend Cat. No. 848801)
AB_2650713 (BioLegend Cat. No. 848802)

Antigen Details

Structure
LC3 is a 125 amino acid protein with a molecular mass of 14.7 kD.
Distribution

Tissue distribution: brain, heart, skeletal muscle, blood and testis.
Cellular distribution: cytosol, mitochondria, cytoskeleton, endoplasmic reticulum, cytoplasmic vesicles, autophagosome.

Function
LC3 proteins play a key role in the selective recruitment of autophagic cargoes into autophagosomes, and serve as docking sites for adaptor proteins.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References

1. Lee J, et al. 2012. Biochem. J. 441(2):523. PubMed
2. Martini-Stoica H, et al. 2016. Trends Neurosci. 39(4)221. PubMed

Gene ID
81631 View all products for this Gene ID
UniProt
View information about LC3 on UniProt.org
Go To Top Version: 1    Revision Date: 01/02/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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