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Purified anti-Lck Phospho (Tyr505) Antibody

Pricing & Availability
Clone
A17013B (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte C-terminal Src kinase, Protein YT16, p56-Lck, T cell-specific protein-tyrosine kinase
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A17013B_PURE_LCK_Phospho_Antibody_WB_091417
Whole cell extracts (15 µg total protein) prepared from Jurkat cells treated with (+) or without (-) 2 mM H202 for three minutes were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL (1:250) purified anti-Lck Phospho (Tyr 505) antibody (Clone A17013B). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG. Equal Lck loading was confirmed using purified anti-Lck antibody used at 1 µg/mL (1:500 dilution).
  • A17013B_PURE_LCK_Phospho_Antibody_WB_091417
    Whole cell extracts (15 µg total protein) prepared from Jurkat cells treated with (+) or without (-) 2 mM H202 for three minutes were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL (1:250) purified anti-Lck Phospho (Tyr 505) antibody (Clone A17013B). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG. Equal Lck loading was confirmed using purified anti-Lck antibody used at 1 µg/mL (1:500 dilution).
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684301 25 µg $112
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684302 100 µg $265
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Description

The Src family tyrosine kinase p56Lck (Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complex results in the recruitment of CD4- and CD8-bound Lck to the TCR/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at Tyrosine 505 negatively regulates Lck, and is proposed to generate a closed, inactive conformation of the protein.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Lck peptide phosphorylated at Tyr 505.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml (1:250-1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is predicted to recognize both 58 and 61 kDa isoforms based off the presence of the immunizing epitope in both proteins. This clone may also recognize certain phosphorylated SRC tyrosine kinase family members due to high sequence similarity.

RRID
AB_2716046 (BioLegend Cat. No. 684301)
AB_2716046 (BioLegend Cat. No. 684302)

Antigen Details

Structure
509 and 539 amino acid isoforms with predicted molecular weights of 58 and 61 kD, respectively. Contains N-terminal domain with myristylation and palmitoylation sites, a kinase domain, and SH2 and SH3 domains that mediate protein-protein interactions bet
Distribution

T cells

Function
T cell receptor signaling
Interaction
AXL, CD2, CD4, CD5, CD8, CD44, CD45, CD122, PI4K, VAV1, RASA1, FYB, CDK1, RAF1, ZAP70, SYK, RUNX3
Cell Type
T cells
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Philipsen L, et al. 2017. Sci Signal. eaaf4736 
2. Moogk D, et al. 2016. J. Immunol. 197:644.
3. Klammt C, et al. 2015. Nat. Immunol. 16:961.
4. Cancer Genome Atlas Network. 2015. Cell. 161:1681.
5. Casas J, et al. 2014. Nat. Commun. 5:5624.
6. McNeill L, et al. 2007. Immunnity. 27:425.
7. Lefebvre DC, et al. 2003. Biochem. Biophys. Acta. 1650:40.
8. Kabouridis PS. 2003. Biochem J. 371:907. 

Gene ID
3932 View all products for this Gene ID
UniProt
View information about Lck Phospho Tyr505 on UniProt.org
Go To Top Version: 0    Revision Date: 09/18/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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