- Clone
- BL28598 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LDHB
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BL28598_Purified_LDHB_Antibody_1_042919.png BL28598_Purified_LDHB_Antibody_1_042919.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BL28598_Purified_LDHB_Antibody_1_042919.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-LDHB antibody (BL28598). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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859801 | 25 µg | $106 |
Lactate Dehydrogenase B (LDHB) is one of the subunits of the lactate dehydrogenase enzyme that catalyzes the synthesis of (S)-Lactate from pyruvate. LDHB was shown to be a downstream mediator of the mTOR signaling pathway that plays a critical role in hyperactive mTOR-mediated tumorigenesis, indicating it as potential therapeutic target for cancer and other diseases caused by aberrant mTOR signaling.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Lactate Dehydrogenase B (LDHB) expressed in E.coli
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2810734 (BioLegend Cat. No. 859801)
Antigen Details
- Structure
- LDHB is a 334 amino acid protein with an apparent molecular mass of ~37 kD.
- Distribution
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Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol - Function
- LDHB is part of the lactate synthesis pathway that synthesizes S-Lactate from pyruvate
- Interaction
- Forms Homotetramers
- Cell Type
- Mature Neurons
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Swiderek K and Paneth P. 2011. Arch. Biochem Biophys. 505(1):33
- Zha X, et al. 2011. Cancer Res. 71(1):13
- Gene ID
- 3945 View all products for this Gene ID 16832 View all products for this Gene ID 24534 View all products for this Gene ID
- UniProt
- View information about LDHB on UniProt.org
Related FAQs
Other Formats
View All LDHB Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-LDHB | BL28598 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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