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Purified anti-LRP1 (CD91) Antibody

Pricing & Availability
Clone
11H4 (See other available formats)
Regulatory Status
RUO
Other Names
LRP1, LDL Receptor Related Protein 1, CD91, APOER
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
11H4_Purified_LRP1_Antibody_031819.png
Western blot of purified anti-LRP1(CD91) antibody (clone 11H4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 11H4_Purified_LRP1_Antibody_031819.png
    Western blot of purified anti-LRP1(CD91) antibody (clone 11H4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
858701 25 µg $106
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858702 100 µg $276
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Description

LRP1 gene encodes a 4544 amino acid precursor protein that is processed in the trans-Golgi complex into the 515 kD alpha chain and the 85 kD beta chain. The alpha and beta chains of LRP1 form a transmembrane receptor on the plasma membrane that plays a role in receptor-mediated endocytosis and lipoprotein metabolism. LRP1 contributes to the maintenance of brain homeostasis through various signaling pathways. It also mediates the uptake and clearance of amyloid beta (Aβ). Dysfunction of LRP1 may be a contributing factor in the developent of  Alzheimer’s disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes the beta chain of LRP1.

Application References

(PubMed link indicates BioLegend citation)
  1. Spoelgen R, et al. 2009. Neuroscience. 158(4):1460 (WB)
  2. Lleó A, et al. 2005. J Biol Chem. 280(29):27303 (WB, ICC)
  3. Campana WM, et al. 2006. J Neurosci. 26(43):11197 (WB, IHC)
  4. Nieves EC and Manchanda N. 2010. J Biol Chem. 285(17):12595 (IP)
RRID
AB_2801223 (BioLegend Cat. No. 858701)
AB_2801223 (BioLegend Cat. No. 858702)

Antigen Details

Structure
The beta chain of LRP1 is a 601 amino acid protein with a molecular mass of 85 kD.
Distribution

Tissue Distribution: brain, lung, adipose tissue, and reproductive system.
Cellular Distribution: plasma membrane, nucleus, cytosol, lysosome, and endosome.

Function
Receptor-mediated endocytosis; lipoprotein metabolism
Interaction
SHC1, GULP1, DAB1, LRPAP1, NPXY
Ligand/Receptor
Receptor for APOE4/APOE, Alpha-2-macroglobulin, Pseudomonas aeruginosa exotoxin A
Cell Type
Microglia, Oligodendrocytes
Biology Area
Cell Motility/Cytoskeleton/Structure, Protein Trafficking and Clearance, Signal Transduction
Molecular Family
Apolipoproteins, Tumor Suppressors
Antigen References
  1. Actis Dato V and Chiabrando GA. 2018. Int J Mol Sci. E1780
  2. Storck SE and Pietrzik CU, 2017. Pharm Res. 34(12):2637
  3. Holtzman DM, et al. 2012, Cold Spring Harb Perspect Med. 2(3):a006312
Gene ID
4035 View all products for this Gene ID 299858 View all products for this Gene ID
UniProt
View information about LRP1 on UniProt.org
Go To Top Version: 1    Revision Date: 03/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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