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Purified anti-MAFB Antibody

Pricing & Availability
Clone
O91E9 (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor MafB, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, MAF bZIP transcription factor B, KRML, MCTO, DURS3,
Isotype
Mouse IgG2b, κ
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Product Citations
publications
O91E9_PURE_MAFB_Antibody_101716
Total protein extracts (15 µg protein) from U87-MG and HepG2 (negative control) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-MAFB (clone O91E9) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
  • O91E9_PURE_MAFB_Antibody_101716
    Total protein extracts (15 µg protein) from U87-MG and HepG2 (negative control) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-MAFB (clone O91E9) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
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692302 25 µg $106
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Description

MAFB belongs to the Maf transcription factor family, the members of which contain basic leucine zipper (bZIP) domains that bind to DNA. These bZIP domains are located within the Maf recognition element (MARE) and regulate the transcription by binding to acidic domains within the target genes. MAFB plays roles in differentiation processes, including the differentiation of macrophage, pancreatic alpha and beta cells, podocytes in the renal glomerulus, and rhombomeres in the embryonic brain. MAFB is also central to embryonic thymus development, parathyroid gland development, the creation of hair cuticles, and urethral masculinization. In the hematopoietic system, MAFB is important for myeloid lineage commitment of hematopoietic stem cells. MAFB was recently reported to have features of oncogene. It was viewed as a sensitive and specific marker for poor prognosis in multiple myeloma patients. Silencing of MAFB inhibited migration and invasion of nasopharyngeal carcinoma cells. In liver cancer, MAFB promotes cancer cell proliferation by transcriptionally targeting Cyclin D1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MAFB protein fragment produced in 293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632763 (BioLegend Cat. No. 692302)

Antigen Details

Structure
323 amino acids with a predicted molecular weight of 36 kD.
Distribution

Nucleus

Function
Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.
Interaction
Homodimer or heterodimer with other bHLH-Zip transcription factors. Binds DNA as a homodimer or a heterodimer. Forms homodimers and heterodimers with FOS, FOSB and FOSL2. Interacts with PAX6, ETS1 and LRP1.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Kataoka K, et al. 1994. Mol. Cell. Biol. 14:700.
2. Eychene A, et al. 2008. Nat. Rev. Cancer 8:683.
3. Miyai M, et al. 2010. J. Dermatol. Sci. 57:178.
4. Suzuki K, et al. 2014. Proc. Natl. Acad. Sci. USA 111:16407.
5. Sarrazin S, et al. 2009. Cell. 138:300.
6. Vicente-Duenas C, et al. 2012. EMBO J. 31:3704.
7. Yang W, et al. 2015. BMC Cancer 15:461.
8. Yu H, et al. 2016. Cell. Physiol. Biochem. 39:700.

Gene ID
9935 View all products for this Gene ID
UniProt
View information about MAFB on UniProt.org

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Go To Top Version: 1    Revision Date: 10/24/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
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