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Purified anti-MAP3K12 Antibody

Pricing & Availability
Clone
N377/20 (See other available formats)
Regulatory Status
RUO
Other Names
Mitogen-Activated Protein Kinase Kinase Kinase 12, DLK, Dual Leucine Zipper Kinase DLK, MAPK-Upstream Kinase, Mixed Lineage Kinase, Leucine Zipper Protein Kinase, MEKK12
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N37720_PURE_MAP3K12_Antibody_110921
Whole tissue membrane extracts (15 µg total protein) from rat and mouse brain were heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-MAP3K12 antibody (clone N377/20) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
  • N37720_PURE_MAP3K12_Antibody_110921
    Whole tissue membrane extracts (15 µg total protein) from rat and mouse brain were heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-MAP3K12 antibody (clone N377/20) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
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608252 100 µg $165
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Description

Mitogen-activated protein kinase kinase kinase 12 (MAP3K12), also known as dual leucine zipper kinase (DLK), is a serine/threonine-protein kinase and part of the non-canonical MAPK signaling pathway. Primarily expressed in neurons at the synaptic terminals, MAP3K12 is a critical regulator of signaling cascades affecting neuronal migration and axon development as well as responding to peripheral nerve injury including transcriptional repression, axon regeneration, and neuronal apoptosis through its activation of transcription factors STAT3 and c-Jun. Its neuroprotective role in responding to injury makes MAP3K12 a promising therapeutic target in the study of neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis (ALS), and optic neuropathy. Emerging evidence suggests a role for MAP3K12 in the proliferation and mass regulation of pancreatic beta cells via activation of c-Jun N-terminal kinase (JNK) signaling pathway and may be a target of interest in the study of the pathogenesis of insulin resistance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 727-888 (C-terminus) of mouse MAP3K12
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has been tested for IHC and failed.

RRID
AB_2904449 (BioLegend Cat. No. 608252)

Antigen Details

Structure
MAP3K12 is an 859 amino acid protein with a predicted molecular weight of 93 kD.
Distribution

Ubiquitously expressed in cell membrane and cytoplasm

Function
Serine/threonine-protein kinase, transferase
Cell Type
Mature Neurons
Biology Area
Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Huntwork-Rodriguez S, et al. 2013. J. Cell Biol. 202:747.
  2. Patel S, et al. 2014. J. Med. Chem. 58:401.
  3. Shin J, et al. 2019. Neurobio. Dis. 127:178.
  4. Tenenbaum M, et al. 2021. Cell. Mol. Life Sci. 78:287.
Gene ID
7786 View all products for this Gene ID
UniProt
View information about MAP3K12 on UniProt.org
Go To Top Version: 1    Revision Date: 11/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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