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Purified anti-ME2 Antibody

Pricing & Availability
Clone
W18113C (See other available formats)
Regulatory Status
RUO
Other Names
Malic Enzyme 2; NAD-Dependent Malic Enzyme, Mitochondrial; Malate Dehydrogenase (Oxaloacetate-Decarboxylating); Malic Enzyme 2, NAD(+)-Dependent, Mitochondrial; NAD-ME; Pyruvic-Malic Carboxylase
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18113C_PURE_ME2_Antibody_1_030524
A-431 cells were stained with 500 nM of MitoSpy™ Green FM (Cat. No. 424805) (panel A) for 30 minutes at 37°C, fixed and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then co-stained with 5.0 µg/mL of Purified anti-ME2 (orange, panel B), followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, merge as panel C). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 60X water objective. Scale bar: 25 µm
  • W18113C_PURE_ME2_Antibody_1_030524
    A-431 cells were stained with 500 nM of MitoSpy™ Green FM (Cat. No. 424805) (panel A) for 30 minutes at 37°C, fixed and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then co-stained with 5.0 µg/mL of Purified anti-ME2 (orange, panel B), followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, merge as panel C). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 60X water objective. Scale bar: 25 µm
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635051 25 µg $140
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635052 100 µg $395
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Description

ME2, also known as malic enzyme 2, is a mitochondrial NAD- or NADP-dependent enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, involving the tricarboxylic acid (TCA) cycle for energy production. During the conversion of malate to pyruvate, ME2 also reduces NAD+ to NADH, participating in cellular respiration and serving as an electron carrier in the electron transport chain. It is a heteromeric protein that plays a crucial role in energy metabolism, cancer, cardiovascular disease, and neurological disorders. ME2 has been found to be upregulated in various types of cancer, including breast, lung, and liver cancer, promoting tumor growth and survival by providing cancer cells with the necessary energy and building blocks for rapid proliferation. Additionally, ME2 assists in glutaminolysis, providing another energy source for cancer cell proliferation. Moreover, P53 has been found to lower the expression of ME2, which induces ROS levels and decreases ATP production, preventing cellular senescence of tumor cells. Furthermore, there may be a link between ME2 and family-associated genetic generalized epilepsy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human ME2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W18113C is not recommended for WB and IHC-P.

For Immunocytochemistry (ICC), ice-cold methanol is recommended for the fixation and permeabilization method.

RRID
AB_3106145 (BioLegend Cat. No. 635051)
AB_3106145 (BioLegend Cat. No. 635052)

Antigen Details

Structure
ME2 has two isoforms due to alternative splicing.
Distribution

Wide tissue expression/mitochondrion matrix

Function
Catalyzes the oxidative decarboxylation of malate to pyruvate/regulate TCA cycle
Interaction
Form homodimer and homotetramer
Cell Type
Leukemia, Neurons, Osteoblasts
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Proliferation and Viability, Mitochondrial Function, Neuroscience, Synaptic Biology
Molecular Family
Enzymes and Regulators, Mitochondrial Markers, Organelle Markers
Antigen References
  1. Pongratz R, et al. 2007. J Biol Chem. 282:200-7.
  2. MacDonald M, et al. 2009. Arch Biochem Biophys. 488:100-4.
  3. Wen Y, et al. 2014. Acta Pharmacol Sin. 35:674-84.
  4. Ren J, et al. 2014. Sci Rep. 4:5414.
  5. Wang Y, et al. 2021. Cell Metab. 33:1027-1041.
  6. Hsieh J, et al. 2023. Commun Biol. 6:548.
  7. Li W, et al. 2023. Proc Natl Acad Sci U S A. 120:e2217869120
Gene ID
4200 View all products for this Gene ID
UniProt
View information about ME2 on UniProt.org
Go To Top Version: 1    Revision Date: 04/04/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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