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Purified anti-MERTK (Mer) Antibody

Pricing & Availability
Clone
A311F9G3E1 (See other available formats)
Regulatory Status
RUO
Other Names
MER Receptor Tyrosine Kinase, c-Mer Proto-Oncogene Tyrosine Kinase, Proto-Oncogene C-Mer, Receptor Tyrosine Kinase MerTK, STK Kinase, MER, RP38, c-Eyk, c-mer, Tyro12, MER-TK
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A311F9G3E1_PURE_MERTK_Antibody_WB_070218
Total cell lysates (15 µg protein) from HepG2 (lane 1), 293E (lane 2), NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-MERTK (Mer) antibody (clone A311F9G3E1) (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-MERTK (Mer) antibody or a donkey anti-rabbit IgG antibody conjugated to HRP for GAPDH (lower panel). Lane M: Molecular weight ladder.
  • A311F9G3E1_PURE_MERTK_Antibody_WB_070218
    Total cell lysates (15 µg protein) from HepG2 (lane 1), 293E (lane 2), NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-MERTK (Mer) antibody (clone A311F9G3E1) (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-MERTK (Mer) antibody or a donkey anti-rabbit IgG antibody conjugated to HRP for GAPDH (lower panel). Lane M: Molecular weight ladder.
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677202 100 µg $311
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Description

MERTK is a receptor tyrosine kinase of the TAM (Tyro3, Axl, MERTK) family, which preferentially is expressed by macrophages, dendritic cells, and natural killer cells. It plays an important role in processes such as macrophage activation, apoptotic cell engulfment, platelet aggregation, and clot stability. MERTK can be activated by dimerization and autophosphorylation through ligand binding or homophilic cell-cell interaction mediated by its NCAM-like motif. The downstream signaling components include PI3 kinase, PLCγ, and MAP kinase. MERTK is upregulated in a wide variety of cancers including leukemia, non-small cell lung cancer, glioblastoma, melanoma, prostate cancer, breast cancer, colon cancer, and gastric cancer.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of recombinant partial-length human MERTK.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Graham DK, et al. 2006. Clin. Cancer Res. 9:2662.
  2. Sather S, et al. 2007. Blood 109:1026.
RRID
AB_2565900 (BioLegend Cat. No. 677202)

Antigen Details

Structure
999 amino acids with a predicted molecular weight of approximately 110 kD with the characteristic of the common NCAM (neural adhesion molecule)-related extracellular domain. Observed molecular weight is 140-160 kD due to different glycosylation patterns.
Distribution

Transmembrane protein. Preferentially expressed by macrophages, dendritic cells, natural killer cells, osteoclasts, Sertoli cells, and retinal pigmental epithelium cells.

Function
Macrophage activation, apoptotic cell engulfment, platelet aggregation, and clot stability.
Interaction
TNK2, LGALS3, TUB, TULP1, GAS6, and VAV1.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References

1. Pierce AM, et al. 2014. Brain Res. 1542:206.
2. Cummings CT, et al. 2013. Clin. Cancer Res. 19:5275.
3. Nguyen KQ, et al. 2013. Autoimmunity 46:294.
4. Qingxian L, et al. 2010. Front Biol. 5:227.
5. Linger RM, et al. 2008. Adv. Cancer Res. 100:35.
6. Lemke G, et al. 2008. Nat. Rev. Immunol. 8:327.
7. Graham DK, et al. 2006. Clin. Cancer Res. 9:2662.
8. Sather S, et al. 2007. Blood 109:1026.

Gene ID
10461 View all products for this Gene ID
UniProt
View information about MERTK on UniProt.org
Go To Top Version: 4    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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