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Purified anti-MFF Antibody

Pricing & Availability
Clone
N382/14 (See other available formats)
Regulatory Status
RUO
Other Names
Mitochondrial Fission Factor, C2orf33, Chromosome 2 Open Reading Frame 33, EMPF2, GL004
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N382-14_Purified_MMF_1_112018
Western blot of purified anti-MFF antibody (clone N382/14). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N382-14_Purified_MMF_1_112018
    Western blot of purified anti-MFF antibody (clone N382/14). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N382-14_Purified_MMF_2_081319
    IHC staining of purified anti-MFF antibody (clone N382/14) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2.0 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • N382-14_Purified_MMF_3_081319
    IHC staining of purified anti-MFF antibody (clone N382/14) on formalin-fixed paraffin-embedded mouse testicle tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2.0 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • N382-14_Purified_MMF_4_081319
    IHC staining of purified anti-MFF antibody (clone N382/14) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2.0 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • N382-14_Purified_MMF_5_081319
    IHC staining of purified anti-MFF antibody (clone N382/14) on formalin-fixed paraffin-embedded rat testicle tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2.0 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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857501 25 µg $83
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857502 100 µg $165
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Description

Mitochondrial fission factor (MFF) is a mitochondrion outer membrane protein that is part of the mitochondrial fission complex where it acts a receptor for the dynamin-related protein 1 (Drp1). The MFF-Drp1 complex is responsible for the promotion of mitochondrial fission. MFF has also been shown to regulate peroxisome morphology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein including amino acids 1-173 and 272-322 of human MFF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Fihurka O, et al. 2023. Biomolecules. 13:. PubMed
  2. Fihurka O, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2783475 (BioLegend Cat. No. 857501)
AB_2783475 (BioLegend Cat. No. 857502)

Antigen Details

Structure
MFF is a 342 amino acid protein with a molecular mass of ~38 kD.
Distribution

Tissue Distribution: Highly expressed in heart, kidney, liver, brain, muscle, and stomach.
Cellular Distribution: Mitochondria, peroxisome, nucleus, and cytosol.

Function
MFF plays a role in mitochondrial and peroxisomal fission by facilitating the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface.
Interaction
MFF interacts with the dynamin-related protein 1 (Drp1). MFF has also been shown to interact with DDX28 and ILF3.
Biology Area
Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience
Molecular Family
Organelle Markers
Antigen References
  1. Moon Yong Cha, et al. 2018. PLoS Curr. 26;10.
  2. Kornfeld OS, et al. 2018. Sci Rep. 19;8(1):14034.
  3. Otera H, et al. 2010. J Cell Biol. 13;191(6):1141-58.
  4. Liu R, et al. 2015. Mol Biol Cell. 1;26(24):4466-77.
Gene ID
56947 View all products for this Gene ID
UniProt
View information about MFF on UniProt.org
Go To Top Version: 2    Revision Date: 08/13/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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