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Purified anti-mMCP-8 Antibody

Pricing & Availability
Clone
TUG8 (See other available formats)
Regulatory Status
RUO
Other Names
Mouse Mast Cell Protease 8, Mcpt8
Isotype
Rat IgG2a, κ
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Product Citations
publications
WB_TUG8_112409
Cell extracts from untransfected NIH/3T3 cells (lane 1) or NIH/3T3 cells transfected with a plasmid encoding mMCP-8-Flag tagged protein (lane 2), using anti-mMCP-8, clone TUG8.
  • WB_TUG8_112409
    Cell extracts from untransfected NIH/3T3 cells (lane 1) or NIH/3T3 cells transfected with a plasmid encoding mMCP-8-Flag tagged protein (lane 2), using anti-mMCP-8, clone TUG8.
Cat # Size Price Quantity Check Availability Save
647401 25 µg $124
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647402 100 µg $293
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Description

mMCP-8 and its rat homologues, rMCP-8, -9, and -10, form a new group of mast cell/basophil proteases, which are more closely related to the T-cell granzymes and neutrophil cathepsin G than to the mast cell tryptases and chymases. mMCP-8 showed a high degree of homology with mouse granzyme B in the critical regions for determining substrate cleavage specificity. It preferentially cleaves after Asp residues. mMCP-8 mRNA is highly expressed in a mouse connective tissue MC-like tumor line. However, it could not be detected in mouse liver, intestine, lung or ears, indicating very low expression in normal tissues. A strong increase in mMCP-8 levels in the lungs can be detected in infected animals.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant GST-mouse mMCP-8 fusion protein expressed in E.coli
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.

Application References

(PubMed link indicates BioLegend citation)
  1. Ugajin T, et al. 2009. J. Leukoc. Biol. 86:1417. (IHC)
  2. Uto-Konomai A, et al. 2012. PLoS One. 7:e40343. PubMed
  3. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  4. Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed
  5. Schwartz C, et al. 2014. PNAS. 111:5169. PubMed
Product Citations
  1. Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed
  2. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  3. Piliponsky AM, et al. 2019. Nat Immunol. 0.922916667. PubMed
  4. Webb LM, et al. 2019. J Exp Med. 216:1268. PubMed
  5. Monte L, et al. 2016. Cancer Res . 76: 1792-1803. PubMed
  6. Sicklinger F, et al. 2021. J Clin Invest. 131:. PubMed
  7. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  8. Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed
  9. Imai Y, et al. 2017. Sci Rep. 10.1038/s41598-017-10227-y. PubMed
RRID
AB_2069309 (BioLegend Cat. No. 647401)
AB_2069309 (BioLegend Cat. No. 647402)

Antigen Details

Structure
A 247-aa polypeptide including a signal sequence of 18 aa followed by a 2-aa activation peptide (Gly-Glu) and a mature protease of 227 aa
Distribution

mMCP-8 was initially isolated as a cDNA from a mouse mast cell line, but has recently been found to be expressed primarily by mouse basophils.

Cell Type
Basophils, Mast cells
Biology Area
Cell Biology, Immunology
Antigen References

1. Lutzelschwab C, et al. 1998. Eur. J. Immunol. 28:1022.
2. Lunderius C, et al. 2001. Immunogenetics 53:225.

Gene ID
4179 View all products for this Gene ID
UniProt
View information about mMCP-8 on UniProt.org
Go To Top Version: 3    Revision Date: 06/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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