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Purified anti-mouse CD115 (CSF-1R) Antibody

Pricing & Availability
Clone
W19330E (See other available formats)
Regulatory Status
RUO
Other Names
CSF-1R, M-CSR, c-fms
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19330E_Purfified_CD115_Antibody_2_021122
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with purified anti-mouse CD115 (clone W19330E) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram) followed by PE goat-anti-rat IgG.
  • W19330E_Purfified_CD115_Antibody_2_021122
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with purified anti-mouse CD115 (clone W19330E) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram) followed by PE goat-anti-rat IgG.
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165202 100 µg $247
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Description

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD115 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W19330E does not affect binding of clone W19330C on target cells.

Clone W19330E can block clone AFS98 binding on target cells.   

Clone W19330E can block ligand binding to CSFR-1.

RRID
AB_2910351 (BioLegend Cat. No. 165202)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts 

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Guilbert LJ and Stanley ER. 1980. J Cell Bio. 85:153-9.
  2. Yeung Y-G, et al. 1978. Proc Natl Acad Sci. 84:1268-71.
  3. Sherr CJ, et al. 1985. Cell. 41:665-76.
Gene ID
12978 View all products for this Gene ID
UniProt
View information about CD115 on UniProt.org
Go To Top Version: 1    Revision Date: 02/11/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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