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Purified anti-mouse CD163 Antibody

Pricing & Availability
Clone
S15049I (See other available formats)
Regulatory Status
RUO
Other Names
M130, MM130, SCARI1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S15049I_Pure_CD163_Antibody_1_043018
C57BL/6 mouse peritoneal macrophages were cultured for seven days in the presence of Dexamethasone, then stained with CD11b APC and purified CD163 (clone S15049I, left), or purified rat IgG2a, κ isotype control (right), followed by PE Goat anti-rat IgG antibody.
  • S15049I_Pure_CD163_Antibody_1_043018
    C57BL/6 mouse peritoneal macrophages were cultured for seven days in the presence of Dexamethasone, then stained with CD11b APC and purified CD163 (clone S15049I, left), or purified rat IgG2a, κ isotype control (right), followed by PE Goat anti-rat IgG antibody.
  • S15049I_Pure_CD163_Antibody_2_043018
    C57BL/6 bone marrow were stained with CD11b APC and purified CD163 (clone S15049I, left), or purified rat IgG2a, κ isotype control (right), followed by PE Goat anti-rat IgG antibody. Data shown was gated on total bone marrow cells.
  • S15049I_Pure_CD163_Antibody_3_102821
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified anti-mouse CD163 Antibody (clone S15049I) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) and CD3e (clone 500A2) Alexa Fluor® 647 (green) for two hours at room temperature. The image was captured with a 10X objective.
  • S15049I_Pure_CD163_Antibody_4_102821
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified anti-mouse CD163 Antibody (clone S15049I) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) for two hours at room temperature. The image was captured with a 10X objective.
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155302 500 µg $293
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD163 extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

 

Product Citations
  1. Pontarollo G, et al. 2023. Nat Metab. 1174:5. PubMed
  2. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
RRID
AB_2734239 (BioLegend Cat. No. 155302)

Antigen Details

Structure
Type 1 transmembrane glycoprotein, 134 kD (non-reduced)/155 kD (reduced) glycoprotein
Distribution

Macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells

Function
Binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
93671 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org
Go To Top Version: 2    Revision Date: 10/28/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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