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- Clone
- R3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- VpreB1, pre-B lymphocyte 1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Pre-B lymphoblast cell line, 70Z/3 was stained with CD179a (clone R3) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 143601 | 25 µg | $65 | ||||
| 143602 | 100 µg | $131 | ||||
CD179a, also known as VpreB, is an 18 kD member of the Ig superfamily, with an Ig V-like domain structure (that lacks the β strand of a typical V domain). CD179a associates with CD179b to form a surrogate light (SL) chain, which associates with the IgM heavy chain and the CD79a/CD79b heterodimer to form the pre-B cell receptor (PreBCR) complex. The function of CD179a/CD179b is still under investigation, though it has been reported that deficiency of CD179a and/or CD179b impairs the maturation of the B cell precursors beyond the pre-B cell stage.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Soluble mouse VpreB protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ELISA, ICC, IP, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western Blotting1, immunoprecipitation1, immunofluorescence staining of 70Z/3 cells1, and ELISA1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Stephan R, et al. 2001. J. Immunol. 167:3734. (ELISA, IF, IP, WB)
- RRID
-
AB_11149492 (BioLegend Cat. No. 143601)
AB_11149492 (BioLegend Cat. No. 143602)
Antigen Details
- Structure
- Ig superfamily, Ig V-like, 18 kD, associates with CD179b to form surrogate light chain
- Distribution
-
Pro and pre-B cells
- Function
- Pre-BCR complex plays a critical role in B cell development
- Cell Type
- B cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stephan R, et al. 2001. J. Immunol. 167:3734.
2. Wang YH, et al. 2002. Blood 99:2459.
3. Martensson IL, et al. 2000. Immunology 101:435. - Gene ID
- 22362 View all products for this Gene ID
- UniProt
- View information about CD179a on UniProt.org
Related FAQs
Other Formats
View All CD179a Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD179a (VpreB) | R3 | FC,ELISA,ICC,IP,WB |
| PE anti-mouse CD179a (VpreB) | R3 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD179a (VpreB)
Pre-B lymphoblast cell line, 70Z/3 was stained with CD179a (... -
PE anti-mouse CD179a (VpreB)
Pre-B lymphoblast cell line, 70Z/3 was stained with CD179a (...
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