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Purified anti-mouse CD200 (OX2) Antibody

Pricing & Availability
Clone
OX-90 (See other available formats)
Regulatory Status
RUO
Other Names
OX-2, MRC
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
OX-90_PURE_CD200_Antibody_101921
C57BL/6 frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then, the tissue was stained with 10 µg/mL of purified anti-mouse CD200 (OX2) Antibody (clone OX-90) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-rat secondary antibody (clone Poly4054) Alexa Fluor® 594 (red) and CD3e (clone 500A2) Alexa Fluor® 647 (green)for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • OX-90_PURE_CD200_Antibody_101921
    C57BL/6 frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then, the tissue was stained with 10 µg/mL of purified anti-mouse CD200 (OX2) Antibody (clone OX-90) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-rat secondary antibody (clone Poly4054) Alexa Fluor® 594 (red) and CD3e (clone 500A2) Alexa Fluor® 647 (green)for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
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123801 50 µg $65
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123802 500 µg $262
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Description

CD200 (OX-2 antigen) is a type-1 membrane glycoprotein containing two extracellular Ig-like domains. CD200 a highly conserved type I membrane glycoprotein that is expressed on a variety of cell types including thymocytes, some T cells, endothelial and follicular dendritc cells, B cells, and brain tissue (neurons); but not on NK cells, granulocytes, monocytes, or macrophages. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. CD200 is the ligand for CD200 receptor (CD200R). The CD200 Receptor is restricted to myeloid cells, and it is believed that its engagement with CD200 results in inhibition and/or downregulation of myeloid cell activity. Blocking of CD200/CD200R interactions decreases myeloid cell inhibitory thresholds which results in enhanced immune activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble fusion protein of the extracellular region of mouse OX-2 antigen with domains 3 and 4 of rat CD4 fusion protein.
Formulation
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Gulati GS, et al. 2018. Nat Protoc. 13:1294. PubMed
  2. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  3. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  4. Menon S, et al. 2021. Nat Commun. 12:4640. PubMed
  5. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
RRID
AB_1236496 (BioLegend Cat. No. 123801)
AB_1236496 (BioLegend Cat. No. 123802)

Antigen Details

Structure
Immunoglobulin (Ig) superfamily
Distribution

Thymocytes, some T cells, endothelial, B cells

Function
Co-stimulatory factor in T cell proliferation
Ligand/Receptor
CD200R
Antigen References

1. Hoek RM, et al. 2000. Science 290:1768.
2. Gorczynski R, et al. 2004. J. of Immunol. 172:7744.
3. Gorczynski L, et al. 1999. J. Immunol. 162:774.
4. Rosenblum MD, et al. 2004. Blood 103:2691.
5. Zhang S, et al. 2004. J. of Immunol. 173:6786.
6. Barclay AN, et al. 2002. Trends Immunol. 23:285.

Gene ID
4345 View all products for this Gene ID
UniProt
View information about CD200 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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