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Purified anti-mouse CD235a (Glycophorin A) Antibody

Pricing & Availability
Clone
S21003A (See other available formats)
Regulatory Status
RUO
Other Names
GPA, CD235a, GYPA, Sialoglycoprotein alpha, MN sialoglycoprotein, PAS-2, Monoclonal antibody to mouse Glycophorin A (GYPA / CD235a)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
S21003A_Pure_Glycophorin-A_Antibody_031022
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD45 FITC and purified anti-mouse CD235a (Glycophorin A) (clone S21003A) (left) or purified rat IgG2b, κ isotype control (right), followed by APC goat anti-rat IgG. Data shown was gated on RBCs and lymphoid together.
  • S21003A_Pure_Glycophorin-A_Antibody_031022
    C57BL/6 mouse bone marrow cells were stained with anti-mouse CD45 FITC and purified anti-mouse CD235a (Glycophorin A) (clone S21003A) (left) or purified rat IgG2b, κ isotype control (right), followed by APC goat anti-rat IgG. Data shown was gated on RBCs and lymphoid together.
  • S21003A_Pure_Glycophorin-A_Antibody_2_031022
    C57BL/6 frozen mouse spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD235a (Glycophorin A) (clone S21003A) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2b (red), 5 µg/mL of Alexa Fluor® 647 anti-mouse/human CD45R/B220 (blue), and Alexa Fluor® 488 anti-mouse CD3ε (green) (left) or Alexa Fluor® 488 anti-mouse F4/80 (green) (right) for two hours at room temperature. The images were captured by 10X objective.
  • S21003A_Pure_Glycophorin-A_Antibody_3_031022
    C57BL/6 frozen mouse submandibular gland section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD235a (Glycophorin A) (clone S21003A) (left) or 5% FBS only (right) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2b (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The images were captured by 10X objective.
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116702 100 µg $317
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Description

CD235a, also known as Glycophorin A (Gypa) is a type I transmembrane sialoglycoprotein that is expressed on mouse erythrocytes, erythroid precursor cells and certain leukemic cells. Glycophorin A has been proposed to be involved in translocation of SLC4A1 to the plasma membrane. Glycophorin A may also be involved in regulation of response to osmotic stress.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Glycophorin A-transfectant cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S21003A does not block the binging of clone Ter-119 to target cells.

Clone S21003A could induce RBCs aggregation at high concentration. 

Clone S21003A reacts with mouse CD235a (Glycophorin A).

 

Product Citations
  1. Chang J, et al. 2023. Research (Wash D C). 6:0120. PubMed
RRID
AB_2924454 (BioLegend Cat. No. 116702)

Antigen Details

Structure
CD235a is type I transmembrane sialoglycoprotein
Distribution

Expressed on mouse erythrocytes, erythroid precursor cells and certain leukemic cells

Function
Regulation of response to osmotic stress. May be involved in translocation of SLC4A1 to the plasma membrane
Cell Type
Erythrocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Arimitsu N, et al. 2003. Genes Cells. 8:769-777-77. 
  2. Devin RB, et al. 2016. Proc Natl Acad Sci USA. 113:5245-50. 
Gene ID
14934 View all products for this Gene ID
UniProt
View information about CD235a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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