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- Clone
- A18209B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T14, S152, Tp55, TNFRSF7
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse splenocytes were stained with purified anti-mouse CD27 (clone A18209B) (left) or purified rat IgG2a, κ isotype control (right), followed by goat anti-rat IgG FITC. Cells were costained with anti-mouse CD3 Brilliant Violet 421™
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 110102 | 100 µg | $112 | ||||
CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, CD27 is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD27 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone A18209B can compete with clone LG.3A10 on target cells.
Clone A18209B can also block binding of CD70 to CD27 in a cell-based binding assay. - RRID
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AB_2910285 (BioLegend Cat. No. 110102)
Antigen Details
- Structure
- TNFR superfamily, 45 kD
- Distribution
-
Peripheral blood T cells, memory B cells, NK cells, thymocytes subset
- Function
- Costimulatory signal for T and B cell activation, T cell development
- Interaction
- TRAF2, TRAF5, CD27-binding protein (Siva)
- Ligand/Receptor
- CD70
- Cell Type
- B cells, Lymphocytes, T cells, Thymocytes
- Biology Area
- Adaptive Immunity, Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Hintzen RQ, et al. 1994. Immunol. Today 15:307-11.
- Akiba H, et al. 1998. J. Biol. Chem. 273:13353-8.
- Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249-53.
- Camerini D, et al. 1991. J. Immunol. 147:3165-9.
- Gene ID
- 21940 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
Other Formats
View All CD27 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD27 | A18209B | FC,Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD27
C57BL/6 mouse splenocytes were stained with purified anti-mo...
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