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- Clone
- W18124D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
BALB/c mice splenocytes were stained with anti-mouse Ly-6G APC and purified anti-mouse CD33 (clone W18124D) (left) or purified rat IgG2a, λ isotype control (right), followed by PE anti-rat IgG2a.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 104802 | 100 µg | $153 | ||||
CD33, also known as Siglec-3, Sigle, gp67, p67, and gp6, is a member of the sialic acid-binding immunoglobulin lectins (Siglec). CD33 extracellular portion contains two immunoglobulin domains (one IgV and one IgC2 domain). The intracellular portion of CD33 contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that are implicated in inhibition of cellular activity. CD33 is primarily expressed on granulocytes in peripheral blood and spleen, as well as on myeloid precursors in the bone marrow.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- RBL1 Transfected with full length mCD33
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2910279 (BioLegend Cat. No. 104802)
Antigen Details
- Structure
- Extracellular portion contains two immunoglobulin domains (IgV and IgC2 domain), intracellular portion contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs)
- Distribution
-
Granulocytes in peripheral blood and spleen, myeloid precursors in the bone marrow
- Function
- Inhibition of cellular activity
- Ligand/Receptor
- Molecule with sialic acid residues
- Cell Type
- Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Siglec Molecules
- Antigen References
-
- Brinkman-Van der Linden, Els C M, et al. 2003. Mol Cell Biol. 23:4199-206.
- Gene ID
- 12489 View all products for this Gene ID
- UniProt
- View information about CD33 on UniProt.org
Other Formats
View All CD33 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD33 | W18124D | FC |
| PE anti-mouse CD33 | W18124D | FC |
| APC anti-mouse CD33 | W18124D | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD33
BALB/c mice splenocytes were stained with anti-mouse Ly-6G A... -
PE anti-mouse CD33
BALB/c mice splenocytes were stained with anti-mouse Ly-6G A... -
APC anti-mouse CD33
BALB/c mice splenocytes were stained with anti-mouse Ly-6G P...
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