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Purified anti-mouse CD3ε Antibody

Pricing & Availability
Clone
500A2 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Syrian Hamster IgG
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Product Citations
publications
A_500A2_PURE_CD3epsilon_Antibody_1_030717
C57BL/6 splenocytes were stained with purified CD3ε (clone 500A2)(top) or purified Syrian Hamster IgG control (bottom) followed by FITC Anti-Goat Syrian hamster IgG, then costained with CD19 (6D5) APC.
  • A_500A2_PURE_CD3epsilon_Antibody_1_030717
    C57BL/6 splenocytes were stained with purified CD3ε (clone 500A2)(top) or purified Syrian Hamster IgG control (bottom) followed by FITC Anti-Goat Syrian hamster IgG, then costained with CD19 (6D5) APC.
  • B_500A2_PURE_CD3epsilon_Antibody_2_030717
  • C_500A2_PURE_CD3epsilon_Antibody_IHCF_040219
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 μg/ml of purified CD3ε (clone 500A2) four hours at room temperature. The staining was followed by 5 μg/ml Goat anti‐hamster (Armenian) IgG (clone Poly4055) Alexa Fluor® 594 (red) as a secondary, and 5 μg/ml of CD45R/B220 (clone RA3‐6B2) Alexa Fluor® 488 (green) at room temperature for two hours. The image was captured with a 10X objective.
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152302 500 µg $242
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
Mouse T cell receptor.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Blocking - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Application References

(PubMed link indicates BioLegend citation)
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113.
Product Citations
  1. Busnelli M, et al. 2022. Arterioscler Thromb Vasc Biol. 42:839. PubMed
RRID
AB_2650621 (BioLegend Cat. No. 152302)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution

Thymocytes (differentiation dependent), mature T cells and NK-T cells.

Function
TCR signal transduction, T cell activation and antigen recognition.
Interaction
T-cell receptor (TCR).
Ligand/Receptor
Peptide antigen/MHC-complex.
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 2    Revision Date: 06/01/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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