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Purified anti-mouse CD8a Antibody

Pricing & Availability
Clone
5H10-1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat (SD) IgG2b, λ
Ave. Rating
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Product Citations
publications
5H10-1_Purified_041106
C57BL/6 mouse splenocytes stained with purified 5H10-1, followed by anti-rat IgGs-FITC
  • 5H10-1_Purified_041106
    C57BL/6 mouse splenocytes stained with purified 5H10-1, followed by anti-rat IgGs-FITC
  • 5H10-1_Purified_CD8a_Antibody_IF_090612
    Mouse OT-I primary CTL cells were stained with purified anti-mouse CD8a primary antibody at 1:50 dilution in PBS/1%NGS incubated O/N at 4°C, detected with anti-rat IgG2b FITC at 1:2000 dilution for 1hr at room temperature (green), plus DAPI staining for nuclei (blue). Imaged with a Zeiss 200M deconvolution microscope (deconvolution applied). Negative control was anti-rat IgG2b isotype control plus secondary antibody, same conditions. Images courtesy of Dr. Javier Casas at The Scripps Research Institute.
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100801 50 µg $83
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100802 500 µg $253
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Concanavalin A-stimulated BALB/c splenic T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, immunohistochemistry of acetone-fixed frozen sections2, and immunofluorescence. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.

Clone 5H10-1 is not recommended for immunohistochemistry of formalin-fixed paraffin sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557.
  2. Frei K, et al. 1997. J. Exp. Med. 185:2177.
Product Citations
  1. Helfen A, et al. 2022. Neoplasia. 28:100792. PubMed
  2. Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
  3. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  4. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
RRID
AB_312763 (BioLegend Cat. No. 100801)
AB_312763 (BioLegend Cat. No. 100802)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org
Go To Top Version: 3    Revision Date: 08/08/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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