- Clone
- 30-H12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Thy-1.2
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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105333 | 100 µg | $183 |
CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
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Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
- Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
- Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
- Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
- Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
- Hardy B, et al. 2005. Int. Immunol. 17:615.
- Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
- Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
- Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
- Product Citations
-
- RRID
-
AB_2563765 (BioLegend Cat. No. 105333)
Antigen Details
- Structure
- Ig superfamily, 25-35 kD
- Distribution
-
Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
- Function
- Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
- Ligand/Receptor
- CD45
- Cell Type
- Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410. - Gene ID
- 21838 View all products for this Gene ID
- UniProt
- View information about CD90.2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD90.2 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with biotinylated 30-H12, f... -
FITC anti-mouse CD90.2 (Thy1.2)
C57BL/6 thymocytes stained with 30-H12 FITC -
PE anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with 30-H12 PE -
Purified anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with purified 30-H12, follo... -
APC anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with 30-H12 APC -
PE/Cyanine5 anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with 30-H12 PE/Cyanine5 -
Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 48... C57BL/6 mouse frozen thymus section was fixed with 4% parafo... -
Alexa Fluor® 647 anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 64... C57BL/6 mouse frozen thymus section was fixed with 4% parafo... -
Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 70... -
PerCP anti-mouse CD90.2 (Thy1.2)
C57BL/6 splenocytes stained with purified 30-H12 PerCP -
Pacific Blue™ anti-mouse CD90.2 (Thy1.2)
Balb/c mouse thymocytes stained with 30-H12 Pacific Blue™ -
PE/Cyanine7 anti-mouse CD90.2 (Thy1.2)
C57BL/6 splenocytes stained with 30-H12 PE/Cyanine7 -
APC/Cyanine7 anti-mouse CD90.2 (Thy1.2)
C57BL/6 splenocytes stained with 30-H12 APC/Cyanine7 -
Brilliant Violet 570™ anti-mouse CD90.2 (Thy1.2)
C57BL/6 splenocytes were stained with CD90.2 (clone 30-H12) ... -
Brilliant Violet 785™ anti-mouse CD90.2 (Thy1.2)
C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) B... -
Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® Ready)
C57BL/6 mouse bone marrow cells stained with 156Gd-anti-CD90... -
Brilliant Violet 510™ anti-mouse CD90.2 (Thy1.2)
C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) B... -
PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2)
C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) P... -
Brilliant Violet 605™ anti-mouse CD90.2 (Thy1.2)
C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) B... -
PE/Dazzle™ 594 anti-mouse CD90.2 (Thy1.2)
C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) P... -
Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2)
C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) B... -
TotalSeq™-A0075 anti-mouse CD90.2 (Thy1.2)
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APC/Fire™ 750 anti-mouse CD90.2 (Thy1.2)
C57BL/6 splenocytes were stained with CD90.2 (clone 30-H12) ... -
Brilliant Violet 711™ anti-mouse CD90.2 (Thy1.2)
C57BL/6 splenocytes were stained with CD90.2 (clone 30-H12) ... -
Ultra-LEAF™ Purified anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse thymocytes stained with LEAF™ purified 30-H12,... -
TotalSeq™-C0075 anti-mouse CD90.2 (Thy1.2)
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TotalSeq™-B0075 anti-mouse CD90.2 (Thy1.2)
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PE/Fire™ 810 anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse splenocytes were stained with anti-mouse CD3e ... -
Spark Blue™ 574 anti-mouse CD90.2 (Thy1.2) (Flexi-Fluor™)
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Spark Red™ 718 anti-mouse CD90.2 (Thy1.2) (Flexi-Fluor™)
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