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Purified anti-mouse DLL4 Antibody

Pricing & Availability
Clone
HMD4-1 (See other available formats)
Regulatory Status
RUO
Other Names
DLL 4
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMD4-1_Pure_021009.jpg
FD4/CHO (mouse DLL4 transfactant) cell line stained with purified HMD4-1, followed by anti-Armenian hamster IgG PE
  • HMD4-1_Pure_021009.jpg
    FD4/CHO (mouse DLL4 transfactant) cell line stained with purified HMD4-1, followed by anti-Armenian hamster IgG PE
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130802 500 µg $393
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Description

The Notch receptors and their ligands are highly conserved from invertebrates to mammals. Delta-like 4 (DLL4) is one of four or five Notch ligands identified. The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. DLL4 is reported to be essential for the regulation of angiogenesis. In thymus, DLL4 is an essential Notch1 ligand responsible for T cell lineage commitment.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cells expressing murine DLL4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Wei Y, et al. 2013. PNAS. 110:3910. PubMed (Block)
Product Citations
  1. Koyanagi A, et al. 2012. Biochem Biophys Res Commun. 418:799. PubMed
  2. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  3. Wei Y, et al. 2013. Proc Natl Acad Sci U S A. 110:3910. PubMed
  4. Jang S, et al. 2010. J Immunol. 185:5835. PubMed
RRID
AB_1227639 (BioLegend Cat. No. 130802)

Antigen Details

Structure
A single-pass transmembrane (TM) protein consisting of a large extracellular N-terminal which includes the DSL domain, followed by a tandem array of EGF-like repeats (ELRs). After the single TM domain is a novel intracellular domain.
Distribution

DLL4 is primarily expressed in arterial blood vessels and sprouting endothelial cells. DLL4 is also expressed in thymic epithelial cells.

Function
The Notch receptors and their ligands are highly conserved signaling molecules throughout development. They regulate cell fate of various cell's development, including myogenesis, neurogenesis, and lymphocyte development.
Ligand/Receptor
Notch Receptors
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Immunology
Antigen References

1. Ehebauer MT, et al. 2006. Biochem J. 392:13.
2. Shimizu K, et al. 2000. Mol Cell Biol. 20:6913.
3. Hellstrom M, et al. 2007. Nature 445:776.
4. Suchting S, et al. 2007. P. Natl. Acad. Sci. USA 104:3225.

Gene ID
54485 View all products for this Gene ID
UniProt
View information about Delta-like 4 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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