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Purified anti-mouse Galectin-9 Antibody

Pricing & Availability
Clone
108A2 (See other available formats)
Regulatory Status
RUO
Other Names
Lgals9, Gal-9, Ecalectin
Isotype
Rat IgG2a, κ
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Product Citations
publications
108A2_PURE_Galectin9_Antibody_1_WB_050316
Western blot analysis of mouse thymus (lane 1), MEF (lane 2), Jurkat (lane 3), and Raji (lane 4) cells using anti-mouse Galectin-9 (clone 108A2) antibody. Purified anti-β-actin (clone Poly6221) was used as loading control.
  • 108A2_PURE_Galectin9_Antibody_1_WB_050316
    Western blot analysis of mouse thymus (lane 1), MEF (lane 2), Jurkat (lane 3), and Raji (lane 4) cells using anti-mouse Galectin-9 (clone 108A2) antibody. Purified anti-β-actin (clone Poly6221) was used as loading control.
  • 108A2_PURE_Galectin9_Antibody_2_IHCF_092016
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse Galectin-9 (clone 108A2) antibody overnight at 4°C followed by 2.5 µg/mL of anti-rat IgG Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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137901 50 µg $101
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137902 500 µg $429
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Description

Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc); Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts and thymus epithelial cells. It may be retained intracellularly or be transported to the cell surface where it is cleaved, thereby generating a soluble form. Galectin-9 is involved in events such as cell aggregation, cell adhesion, chemotaxis and apoptosis. Importantly for the regulation of the immune response, Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length recombinant mouse Galectin-9 (M-type)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICFC, IHC-F, WB - Verified
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. (ELISA)
  2. Personal communication. (IHC-Paraffin)
  3. Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
  4. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
Product Citations
  1. Oh J, et al. 2022. Int J Mol Sci. 23:. PubMed
  2. Sudhakar JN, et al. 2020. Nat Commun. 3.434722222. PubMed
  3. Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
  4. Smith LK, et al. 2021. Elife. 10:. PubMed
  5. Rebelo C, et al. 2022. Nat Commun. 13:4135. PubMed
  6. Baloche V, et al. 2021. Scientific Reports. 11(1):5227. PubMed
RRID
AB_10568691 (BioLegend Cat. No. 137901)
AB_10568691 (BioLegend Cat. No. 137902)

Antigen Details

Structure
Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution

Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells

Function
Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand/Receptor
Tim-3
Cell Type
Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Klibi J, et al. 2009. Blood 113:1957.
2. Seki M, et al. 2008. Clin. Immunol. 127:78.
3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
4. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.

Gene ID
16859 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 09/20/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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