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Purified anti-mouse GITR Ligand Antibody

Pricing & Availability
Clone
5F1 (See other available formats)
Regulatory Status
RUO
Other Names
GITRL, AITRL, TNFSF18, TL6
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5F1_Purified_GITRL_Antibody_FC_042414
GITR ligand transfected cells were stained with purified GITR ligand (clone 5F1, filled histogram) or purified rat IgG2a, κ isotope control (open histogram), followed by anti-rat IgG PE.
  • 5F1_Purified_GITRL_Antibody_FC_042414
    GITR ligand transfected cells were stained with purified GITR ligand (clone 5F1, filled histogram) or purified rat IgG2a, κ isotope control (open histogram), followed by anti-rat IgG PE.
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147402 100 µg $265
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Description

GITR ligand (GITRL) is a member of the TNF superfamily, also known as TNFSF18 or TL6. It is expressed on B cells, macrophages, dendritic cells, and endothelial cells. The expression of GITRL on the dendritic cell surface can be transiently upregulated, and then rapidly downregulated, by LPS stimulation. GITRL binds to its receptor, GITR, to costimulate T cell activation, proliferation, and cytokine production, inhibit the suppressive functions of CD4+CD25+ Tregs, and induce macrophage activation. GITR-GITRL interaction has been implicated as an essential regulator of the immune response to self antigens and phathogens.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additonal reported applications (for relevant formats of thes clone) include: inhibition of GITR-GITRL mediated T cell proliferation and reversing Treg-mediated suppression1.

The following patents apply to clone 5F1: U.S. Patent Serial No. 7,618,632 and Japan Patent Serial No. 4638876.

Application References

(PubMed link indicates BioLegend citation)
  1. Stephens GL, et al. 2004. J. Immunol. 173:5008. (Block)
RRID
AB_2563295 (BioLegend Cat. No. 147402)

Antigen Details

Structure
TNF superfamily member
Distribution
B cells, macrophages, dendritic cells, endothelial cells
Function
Binds GITR receptor to costimulate T cell activation, proliferation, and cytokine production; inhibits the suppressive activities of CD4+CD25+ Tregs
Cell Type
B cells, Macrophages, Dendritic cells, Endothelial cells, Tregs
Biology Area
Immunology
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
4. Shin HH, et al. 2002. Cytokine 19:187.

Gene ID
240873 View all products for this Gene ID
UniProt
View information about GITR Ligand on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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