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Purified anti-mouse H-2Dk Antibody

Pricing & Availability
Clone
15-5-5 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (C3H.SW) IgG2a, κ
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Product Citations
publications
15.5.5_Pure_112911
C3H/He mouse splenocytes were stained with purified H-2Dk (clone 15-5-5) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a FITC.
  • 15.5.5_Pure_112911
    C3H/He mouse splenocytes were stained with purified H-2Dk (clone 15-5-5) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a FITC.
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110302 500 µg $253
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Description

The 15-5-5 antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1.

This clone does not cross react with other haplotypes (e.g., a, b, p, q, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1980. J. Immunol. 124:533.
  2. Yamamoto M, et al. 1998. J. Immunol. 160:2188.
  3. Houlden BA, et al. 1991. J. Immunol. 146:425. (IP) PubMed
Product Citations
  1. Fodil-Cornu N, et al. 2011. PLoS One. 6:e1001368. PubMed
RRID
AB_313475 (BioLegend Cat. No. 110302)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Dk haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
UniProt
View information about H-2Dk on UniProt.org
Go To Top Version: 2    Revision Date: 08/11/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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