Loading...

Purified anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
IM7_Pure_091907
C57BL/6 mouse splenocytes stained with purified IM7, followed by anti-rat IgG FITC
  • IM7_Pure_091907
    C57BL/6 mouse splenocytes stained with purified IM7, followed by anti-rat IgG FITC
  • IM7_pure_CD44_Antibody_2_IHC_102418
    Fresh, frozen mouse spleen was stained with purified CD44 clone IM7 conjugated and detected with a Cy3 CODEX™ oligonucleotide duplex (red). Samples were counterstained with CollagenIV Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Compare all formats
Cat # Size Price Quantity Check Availability Save
103001 50 µg $42
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103002 500 µg $138
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, CyTOF® - Verified
ELISA, ICC, IHC-P, IP, CMCD, Stim - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  2. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  3. Villafranca C, et al. 2020. Cytotechnology. . PubMed
  4. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  5. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  6. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  7. Wangzhou K, et al. 2023. Oral Dis. 29:491. PubMed
  8. Campisi L, et al. 2022. Nature. 606:945. PubMed
  9. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  10. Ren L, et al. 2023. iScience. 26:105769. PubMed
  11. Alnajar H, et al. 2022. Cold Spring Harb Mol Case Stud. 8:. PubMed
  12. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  13. Huang R, et al. 2023. Adv Sci (Weinh). 10:e2206542. PubMed
  14. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  15. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  16. Reeves PM, et al. 2017. Oncotarget. 8:94040. PubMed
  17. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  18. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  19. Emmerson E et al. 2017. eLife. 6 pii: e26620. PubMed
  20. Min CK, et al. 2018. Front Immunol. 1.779166667. PubMed
  21. Dubrot J, et al. 2018. Life Sci Alliance. 1:e201800164. PubMed
  22. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  23. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  24. He N 2005. J Immunol. 174:6967. PubMed
  25. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
  26. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  27. Haque M, et al. 2021. Viruses. 13:. PubMed
  28. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  29. Son YM, et al. 2020. Eur J Immunol. 50:1067. PubMed
  30. Dertschnig S, et al. 2020. J Clin Invest. 130:1896. PubMed
  31. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  32. Walker F, et al. 2011. PLoS One. 6:e22733. PubMed
  33. Yamazaki J, et al. 2009. Blood. 114:2709. PubMed
  34. Frank N, et al. 2005. Cancer Res. 65:4320. PubMed
  35. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  36. Noguerol J, et al. 2019. Biol Sex Differ. 10:47. PubMed
  37. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  38. Hirokawa Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:547. PubMed
  39. Lee JH, et al. 2022. iScience. 25:104166. PubMed
  40. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  41. Delgobo M, et al. 2021. Front Immunol. 12:584538. PubMed
  42. Muhammad F, et al. 2020. Front Immunol. 975:11. PubMed
  43. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  44. Kaisar MMM, et al. 2018. PLoS Biol. 16:e2005504. PubMed
  45. Taylor NA, et al. 2017. J Clin Invest. 127:3472. PubMed
  46. Morabito KM, et al. 2017. Mucosal Immunol. 0.795138889. PubMed
  47. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  48. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  49. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  50. Fiege JK, et al. 2019. PLoS Pathog. 15:e1008077. PubMed
  51. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  52. Pace JA, et al. 2021. Sci Adv. 7:eabg6600. PubMed
  53. Ghanei Z, et al. 2020. Journal of Cellular Physiology. 235:7840. PubMed
  54. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  55. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  56. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  57. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  58. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  59. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  60. Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed
  61. Sato Y, et al. 2021. BMC Cancer. 21:1222. PubMed
  62. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  63. Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
  64. Khairnar V, et al. 2018. Nat Commun. 2.153472222. PubMed
  65. Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
  66. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  67. Wang D, et al. 2019. Cancers (Basel). 11. PubMed
  68. Emmerson E, et al. 2018. EMBO Mol Med. 10.15252/emmm.201708051. PubMed
  69. Zhou Z, et al. 2018. Cancer Res. 78:2248. PubMed
  70. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  71. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  72. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  73. You X, et al. 2022. Front Genet. 12:790990. PubMed
  74. Heng WS, et al. 2021. Lung Cancer. 154:13. PubMed
  75. Tang F, et al. 2015. Sci Signal. 8: ra100. PubMed
  76. Kenna T, et al. 2008. Blood. 111:2091. PubMed
  77. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  78. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  79. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  80. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  81. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  82. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  83. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  84. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  85. Klingler S, et al. 2022. JCI Insight. 7:. PubMed
  86. Flietner E, et al. 2022. Sci Rep. 12:10616. PubMed
  87. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  88. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  89. Meyers JL, et al. 2018. PLoS One. 13:e0207007. PubMed
  90. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  91. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  92. Yi J, et al. 2019. Mol Cells. 42:228. PubMed
  93. Wang X, et al. 2008. Blood. 111:2436. PubMed
  94. Heijink A, et al. 2015. Proc Natl Acad Sci U S A. 112: 15160 - 15165. PubMed
  95. Arkatkar T, et al. 2018. Kidney Int. 94:728. PubMed
  96. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  97. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  98. Jun JC, et al. 2017. PLoS One. 12:e0180688. PubMed
  99. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  100. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  101. Nguyen H, et al. 2020. Clin Cancer Res. 26:3481. PubMed
  102. Karuppuchamy T, et al. 2020. Inflamm Bowel Dis. 216:26. PubMed
  103. Gui J, et al. 2007. Int Immunol. 1.625694444. PubMed
  104. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  105. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  106. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
  107. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  108. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  109. Bordoloi D, et al. 2021. Genes Cancer. 12:51. PubMed
  110. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  111. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
  112. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
  113. Guo Y, et al. 2018. J Am Soc Nephrol. 29:2529. PubMed
RRID
AB_312952 (BioLegend Cat. No. 103001)
AB_312952 (BioLegend Cat. No. 103002)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC,ICC,IHC-P
FITC anti-mouse/human CD44 IM7 FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC,SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Brilliant Violet 750™ anti-mouse/human CD44 IM7 FC
PerCP/Fire™ 806 anti-mouse/human CD44 IM7 FC
Spark Red™ 718 anti-mouse/human CD44 IM7 FC
PE/Fire™ 810 anti-mouse/human CD44 IM7 FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Go To Top Version: 4    Revision Date: 10/24/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account